SM1693PS 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €260.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti 6xHistidine Epitope Tag AD1.1.10

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation Purified
Applications C, E, IP, WB
Clonality Monoclonal
Clone AD1.1.10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

PAX6 transcription factor linked to Histidine tag.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Isotype control AM03095PU-N
Application ELISA.
Immunhistology on Frozen Sections.
Western blot
(1/500-1/1000): This antibody is routinely tested on Histidine tagged recombinant protein.
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Papadaki A, Politou AS, Smirlis D, Kotini MP, Kourou K, Papamarcaki T, et al. The Leishmania donovani histidine acid ecto-phosphatase LdMAcP: insight into its structure and function. Biochem J. 2015 Feb 19. PubMed PMID: 25695743.
General Readings
  1. Els Conrath K, Lauwereys M, Wyns L, Muyldermans S. Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J Biol Chem. 2001 Mar 9;276(10):7346-50. Epub 2000 Oct 25. PubMed PMID: 11053416.
  2. Bahi A, Boyer F, Kafri T, Dreyer JL. CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur J Neurosci. 2004 Mar;19(6):1621-33. PubMed PMID: 15066158.
  3. Hoffmann SC, Schellack C, Textor S, Konold S, Schmitz D, Cerwenka A, et al. Identification of CLEC12B, an inhibitory receptor on myeloid cells. J Biol Chem. 2007 Aug 3;282(31):22370-5. Epub 2007 Jun 11. PubMed PMID: 17562706.
  4. Zheng J, Kohler ME, Chen Q, Weber J, Khan J, Johnson BD, et al. Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen. BMC Immunol. 2007 Mar 30;8:4. PubMed PMID: 17397536. (Free PMC Article available, 6 images available)
  5. Bahi A, Dreyer JL. Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization. Genes Brain Behav. 2008 Mar;7(2):244-56. Epub 2007 Aug 3. PubMed PMID: 17680802.
  6. Wrighton KH, Lin X, Yu PB, Feng XH. Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors. J Biol Chem. 2009 Apr 10;284(15):9755-63. doi: 10.1074/jbc.M809223200. Epub 2009 Feb 18. PubMed PMID: 19224917. (Free PMC Article available, 9 images available)
  7. Diefenbacher M, Thorsteinsdottir H, Spang A. The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae. J Biol Chem. 2011 Jul 15;286(28):25027-38. doi: 10.1074/jbc.M110.215657. Epub 2011 Apr 11. PubMed PMID: 21482823. (Free PMC Article available, 5 images available)
  8. Alvarez MM, López-Pacheco F, Aguilar-Yañez JM, Portillo-Lara R, Mendoza-Ochoa GI, García-Echauri S, et al. Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. PLoS One. 2010 Apr 14;5(4):e10176. doi: 10.1371/journal.pone.0010176. PubMed PMID: 20418957. (Free PMC Article available, 10 images available)
  9. Bahi A, Kusnecov A, Dreyer JL. The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement. Neuropsychopharmacology. 2008 Oct;33(11):2726-34. doi: 10.1038/sj.npp.1301668. Epub 2008 Feb 6. PubMed PMID: 18256596.
  10. Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ. Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnol. 2010 Feb 3;10:8. doi: 10.1186/1472-6750-10-8. PubMed PMID: 20128915. (Free PMC Article available, 6 images available)
  11. Hwang HY, Duvall MR, Tomlinson S, Boackle RJ. Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment. Mol Immunol. 2008 May;45(9):2570-80. doi: 10.1016/j.molimm.2007.12.019. Epub 2008 Mar 3. PubMed PMID: 18313756. (Free PMC Article available, 8 images available)
  12. De Vooght, L. et al. (2012) Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly. Microb Cell Fact. 11: 23.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4
0.09% Sodium Azide
Liquid purified IgG fraction
This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. We recommend the use of SM1693P for this application.

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