AR09474PU-N Arginase-1 (1-322, His-tag)

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0.1 mg / €290.00
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Arginase-1

Product Description for Arginase-1

Arginase-1.
Presentation: Purified

Properties for Arginase-1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms ARG1, Liver-type arginase, Type I arginase
Presentation Purified
Molecular weight 35.8 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05089
Purity > 85 % by SDS - PAGE
Background Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings
  1. Iyer RK, Yoo PK, Kern RM, Rozengurt N, Tsoa R, O'Brien WE, et al. Mouse model for human arginase deficiency. Mol Cell Biol. 2002 Jul;22(13):4491-8. PubMed PMID: 12052859. (Free PMC Article available, 5 images available)
  2. Wu G., et al (1998) The Biochemical journal 336 (Pt 1) 1-17.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human ARG1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH
Molecular weight:
(330 aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2 mM DTT, 100 mM NaCl
Purity:
>85% by SDS - PAGE
State:
Liquid purified protein
Purified
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