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TRAF1 / EBI6 antibody

Principal name

TRAF1 / EBI6 antibody

Alternative names for TRAF1 / EBI6 antibody

TNF receptor-associated factor 1, Epstein-Barr virus-induced protein 6

SwissProt ID

P39428 (Mouse), Q13077 (Human)

Gene ID

7185 (TRAF1)

Ncbi ID

9606, NP_005649, NP_001177874.1, 10090, AAH24145, NM_005658

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of TRAF1 / EBI6 antibody

This protein is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the sigl transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic sigls from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the sigl transduction from TNFR family receptors.The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the sigl transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic sigls from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the sigl transduction from TNFR family receptors. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Leo E,et al. (2001) J Biol Chem. 276(11):8087-93.

Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF2 and TRAF1 HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-TRAF1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00007185-M01

Formalin-fixed, paraffin-embedded human lymphoma tissue section stained for TRAF1 expression. Using TRAF1 polyclonal antibody ( Cat # PAB0274 ) at 1 : 2000. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.

PAB0274

Western blot analysis of TRAF1 in normal human tissues. Using TRAF1 polyclonal antibody ( Cat # PAB0274 ) at 1 : 2000. TRAF1 is observed at 50-50 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF1 degradation fragments.

PAB0274

TRAF1 polyclonal antibody ( Cat # PAB6988 ) ( 0.5 µg/ml ) staining of U-937 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6988

Immunoperoxidase of purified MaxPab antibody to TRAF1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

H00007185-B01

Western Blot analysis of TRAF1 expression in transfected 293T cell line (H00007185-T01) by TRAF1 MaxPab polyclonal antibody.Lane 1: TRAF1 transfected lysate(45.76 KDa).Lane 2: Non-transfected lysate.

H00007185-B01

Immunoperoxidase of purified MaxPab antibody to TRAF1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

H00007185-B01P

Western Blot analysis of TRAF1 expression in transfected 293T cell line (H00007185-T01) by TRAF1 MaxPab polyclonal antibody.Lane 1: TRAF1 transfected lysate(45.76 KDa).Lane 2: Non-transfected lysate.

H00007185-B01P

Western Blot analysis of TRAF1 expression in transfected 293T cell line (H00007185-T02) by TRAF1 MaxPab polyclonal antibody.Lane 1: TRAF1 transfected lysate(46.20 KDa).Lane 2: Non-transfected lysate.

H00007185-D01P

Immunoprecipitation of TRAF1 transfected lysate using anti-TRAF1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TRAF1 MaxPab mouse polyclonal antibody (B01) (H00007185-B01).

H00007185-D01

Recombinant human TRAF1, 266-416 aa, His-tagged

Human 293T; WB Suggested Anti-TRAF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; TRAF1 antibody - middle region (AVARP09008_P050) in Human 293T cells using Western Blot

AVARP09008_P050

Western blot analysis of Mouse TRAF1 in mouse kidney (1) and mouse liver (2) using AM26584AF-N.

AM26584AF-N

TP302418

TA303092 (0.5µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303092

Predicted band size: 46 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 second)

TA324108

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using TRAF1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)

TA324108

Predicted band size: 46 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)

TA324109

Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using TRAF1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)

TA324109

WB Suggested Anti-TRAF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate

TA330395

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA303092	TRAF1 / EBI6 antibody
	Goat Polyclonal Antibody against TRAF1
	Goat			Aff - Purified	Hu, Ms	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA324108	TRAF1 / EBI6 antibody
	Anti-TRAF1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA324109	TRAF1 / EBI6 antibody
	Anti-TRAF1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
AM26584AF-N	TRAF1 / EBI6 (1-410) antibody
	Mouse	IgG1	3D4	Azide Free	Ms	WB	0.1 mg / €395.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP302418	TRAF1 / EBI6
	Recombinant protein of human TNF receptor-associated factor 1 (TRAF1)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY417149	TRAF1 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

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