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TRIM22 / RNF94 antibody

Principal name

TRIM22 / RNF94 antibody

Alternative names for TRIM22 / RNF94 antibody

STAF50, Staf-50, RING finger protein 94, Tripartite motif-containing protein 22, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor

SwissProt ID

Q8IYM9 (Human)

Gene ID

10346 (TRIM22)

Ncbi ID

9606, NP_006065, NP_001186502.1, NM_006074

Available reactivities

Hu (Human), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TRIM22 / RNF94 antibody

TRIM22 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. It localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon\'s antiviral effects. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon\'s antiviral effects. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Obad,S., (2007) J. Interferon Cytokine Res. 27 (10), 857-864

Western Blot analysis of TRIM22 expression in transfected 293T cell line (H00010346-T01) by TRIM22 MaxPab polyclonal antibody.Lane 1: TRIM22 transfected lysate(54.78 KDa).Lane 2: Non-transfected lysate.

H00010346-B01P

Human Brain; WB Suggested Anti-TRIM22 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; TRIM22 antibody - N-terminal region (P100888_P050) in Human Brain cells using Western Blot

P100888_P050

Transfected 293T; WB Suggested Anti-TRIM22 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; TRIM22 antibody - middle region (ARP38734_P050) in Transfected 293T cells using Western Blot

ARP38734_P050

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505253)

TA505253

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505253)

TA505253

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM22 (RC207431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM22.

TA505253

E.coli lysate (5 ug, left lane) and E.coli lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 61-406 of human TRIM22 (NP_006065) were separated by SDS-PAGE and immunoblotted with anti-TRIM22.

TA505278

TA505281

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505281)

TA505281

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505281)

TA505281

TA505281

Anti-TRIM22 mouse monoclonal antibody (TA505281) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM22(RC207431).

TA505281

TA505281

TA505295

WB Suggested Anti-TRIM22 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain

TA330115

WB Suggested Anti-TRIM22 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T

TA343580

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA505281	TRIM22 / RNF94 antibody
	TRIM22 mouse monoclonal antibody, clone OTI1D11 (formerly 1D11)
	Mouse	IgG1	OTI1D11	Purified	Hu	ICC/IF, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
TA351809	TRIM22 / RNF94 antibody
	Rabbit Polyclonal Anti-TRIM22 Antibody
	Rabbit	IgG		Purified	Hu	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA505253	TRIM22 / RNF94 antibody
	TRIM22 mouse monoclonal antibody, clone OTI1D3 (formerly 1D3)
	Mouse	IgG1	OTI1D3	Purified	Hu	P, WB	0.1 ml / €360.00
							OriGene Technologies, Inc.
+2 additional images
TA505278	TRIM22 / RNF94 antibody
	TRIM22 mouse monoclonal antibody, clone OTI2G2 (formerly 2G2)
	Mouse	IgG1	OTI2G2	Purified	Hu	WB	0.1 ml / €360.00
							OriGene Technologies, Inc.

TA505295	TRIM22 / RNF94 antibody
	TRIM22 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)
	Mouse	IgG2a	OTI1A10	Purified	Hu	WB	0.1 ml / €360.00
							OriGene Technologies, Inc.

AP54907PU-N	TRIM22 / RNF94 antibody
	Rabbit			Purified	Hu	C, E, WB	0.1 mg / €450.00
							OriGene Technologies GmbH

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