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TRIM32 / HT2A antibody

Principal name

TRIM32 / HT2A antibody

Alternative names for TRIM32 / HT2A antibody

Tripartite motif-containing protein 32, Zinc finger protein HT2A, 72 kDa Tat-interacting protein

SwissProt ID

Q13049 (Human), Q8CH72 (Mouse)

Gene ID

22954 (TRIM32), 69807 (Trim32)

Ncbi ID

9606, NP_036342, NP_001093149, NP_001093149.1, NP_001155254.1, NM_001161782, NM_053084, NM_012210, NM_001099679

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TRIM32 / HT2A antibody

TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

General readings

Chiang,A.P., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (16), 6287-6292

Western Blot analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5.Lane 1: TRIM32 transfected lysate(72 KDa).Lane 2: Non-transfected lysate.

H00022954-M09

Western blot analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi ( Cat # H00022954-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM32 monoclonal antibody (M09), clone 2E5 (Cat # H00022954-M09 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00022954-M09

Detection limit for recombinant GST tagged TRIM32 is approximately 0.3ng/ml as a capture antibody.

H00022954-M09

Human Jurkat; WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; TRIM32 antibody - N-terminal region (ARP38965_T100) in Human Jurkat cells using Western Blot

ARP38965_T100

Human Bronchial Epithelial Tissue; Rabbit Anti-TRIM32 Antibody . Catalog Number: ARP31692_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Cytoplasmic . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-TRIM32 Antibody (ARP31692_P050) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry

ARP31692_P050

Human 293T; WB Suggested Anti-TRIM32 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T. TRIM32 is supported by BioGPS gene expression data to be expressed in HEK293T.; TRIM32 antibody - C-terminal region (ARP31692_P050) in Human 293T cells using Western Blot

ARP31692_P050

Western Blot analysis of TRIM32 expression in transfected 293T cell line (H00022954-T01) by TRIM32 MaxPab polyclonal antibody.Lane 1: TRIM32 transfected lysate(71.83 KDa).Lane 2: Non-transfected lysate.

H00022954-B01P

Human Muscle; TRIM32 antibody - C-terminal region (ARP38966_T100) in Human Muscle cells using Immunohistochemistry

ARP38966_T100

Human 293T; WB Suggested Anti-TRIM32 Antibody Titration: 1.025 ug/ml. Positive Control: 293T cells lysate. TRIM32 is supported by BioGPS gene expression data to be expressed in HEK293T.; TRIM32 antibody - C-terminal region (ARP38966_T100) in Human 293T cells using Western Blot

ARP38966_T100

Immunoprecipitation of TRIM32 transfected lysate using anti-TRIM32 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TRIM32 MaxPab mouse polyclonal antibody (B01) (H00022954-B01).

H00022954-D01

Human Bronchial Epithelial Tissue; Rabbit Anti-TRIM32 Antibody . . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Cytoplasmic . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-TRIM32 Antibody (AP42053PU-N) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry

AP42053PU-N

AP42053PU-N

TP301289

TP323796

TRIM32 antibody detects TRIM32 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. 7.5 % SDS-PAGE. TRIM32 antibody (TA308451) dilution: 1:1000

TA308451

TRIM32 antibody detects TRIM32 protein by western blot analysis. A. 5 ug 293T whole cell lysate/extract. B. 5 ug whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells. 7.5 % SDS-PAGE. TRIM32 antibody (TA308451) dilution: 1:20000

TA308451

TRIM32 antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: TRIM32 protein stained by TRIM32 antibody (TA308451) diluted at 1:500. Blue: Hoechst 33342 staining. Scale

TA308451

Predicted band size: 72 kDa . Positive control: Jurkat, PC3 and Lovo cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Jurkat cell lysate Lane2: PC3 cell lysate Lane3: Lovo cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)

TA323256

Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using TRIM32 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)

TA323256

Predicted band size: 72 kDa . Positive control: Lovo cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes)

TA323257

Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using TRIM32 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)

TA323257

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA308451	TRIM32 / HT2A antibody
	Rabbit polyclonal antibody to TRIM32 (tripartite motif-containing 32)
	Rabbit	IgG	Aff - Purified	Hu, Rt	ICC/IF, P, WB	0.1 ml / €415.00
						OriGene Technologies, Inc.
+2 additional images
TA323256	TRIM32 / HT2A antibody
	Anti-TRIM32 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA323257	TRIM32 / HT2A antibody
	Anti-TRIM32 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image

CRISPR

Catalog No.	Species	Donor Vector
KN318203	Trim32 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN318203BN	Trim32 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN318203LP	Trim32 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN318203RB	Trim32 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP301289	TRIM32 / HT2A (transcript variant 1)
	Recombinant protein of human tripartite motif-containing 32 (TRIM32), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

TP323796	TRIM32 / HT2A (transcript variant 2)
	Purified recombinant protein of Homo sapiens tripartite motif-containing 32 (TRIM32), transcript variant 2
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR209801L2V	Lenti ORF particles, Trim32 (GFP-tagged) - Mouse tripartite motif-containing 32 (Trim32), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,240.00
		OriGene Technologies, Inc.

MR209801L1V	Lenti ORF particles, Trim32 (Myc-DDK-tagged) - Mouse tripartite motif-containing 32 (Trim32), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,110.00
		OriGene Technologies, Inc.

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