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Home » Target » UBE2C / UBCH10 antibody

UBE2C / UBCH10 antibody

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Principal name

UBE2C / UBCH10 antibody

Alternative names for UBE2C / UBCH10 antibody

Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, Ubiquitin carrier protein C

SwissProt ID

O00762 (Human), Q32PA5 (Bovin), Q9D1C1 (Mouse)

Gene ID

11065 (UBE2C), 68612 (Ube2c)

Ncbi ID

NP_008950.1, NP_008950, NM_181799, 9606, NM_181801, NP_861517, NM_007019, NM_181803, NP_081061.1

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of UBE2C / UBCH10 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human UBE2C with an internal ID of P25065

General readings

Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV.
Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase.
Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7
PMID: 9122200

AP17818PU-N - UBE2C / UBCH10
Western blot analysis of UBE2C (arrow) using rabbit polyclonal UBE2C Antibody (N-term G25) (AP17818PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2) (Origene Technologies).(8ug/ml)
ARP43090_P050 - UBE2C / UBCH10
Human Muscle; WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; UBE2C antibody - N-terminal region (ARP43090_P050) in Human Muscle cells using Western Blot
ARP43091_P050 - UBE2C / UBCH10
Human Muscle; WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; UBE2C antibody - middle region (ARP43091_P050) in Human Muscle cells using Western Blot
ARP43474_P050 - UBE2C / UBCH10
Human THP-1; WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: THP-1 cell lysate; UBE2C antibody - middle region (ARP43474_P050) in Human THP-1 cells using Western Blot
H00011065-A02 - UBE2C / UBCH10
UBE2C polyclonal antibody (A02), Lot # 051122JC01 Western Blot analysis of UBE2C expression in IMR-32 ( Cat # L008V1 ).
H00011065-M01 - UBE2C / UBCH10
Immunoperoxidase of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
H00011065-M01 - UBE2C / UBCH10
Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell. [antibody concentration 10 ug/ml]
H00011065-M01 - UBE2C / UBCH10
UBE2C monoclonal antibody (M01), clone 9D3 Western Blot analysis of UBE2C expression in HeLa ( Cat # L013V1 ).
H00011065-M01 - UBE2C / UBCH10
Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M01), clone 9D3.Lane 1: UBE2C transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
H00011065-M01 - UBE2C / UBCH10
Detection limit for recombinant GST tagged UBE2C is approximately 0.03ng/ml as a capture antibody.
H00011065-M04 - UBE2C / UBCH10
Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M04), clone 3B1.Lane 1: UBE2C transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
AR50505PU-N - UBE2C / UBCH10
TP300240 - UBE2C / UBCH10
TP308741 - UBE2C / UBCH10
TA329810 - UBE2C / UBCH10
WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle
TA329811 - UBE2C / UBCH10
WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle
TA331549 - UBE2C / UBCH10
WB Suggested Anti-UBE2C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: THP-1 cell lysate
TA311373 - UBE2C / UBCH10
(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA332727 - UBE2C / UBCH10
Western blot analysis of extracts of various cell lines, using UBE2C antibody.
TA332727 - UBE2C / UBCH10
Immunofluorescence analysis of HeLa cell using UBE2C antibody. Blue: DAPI for nuclear staining.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UBE2C / UBCH10 (C-term) antibody
AP16056PU Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). Goat Aff - Purified Hu E, F, ICC/IF, IP, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

UBE2C / UBCH10 antibody
  Goat Anti-UBE2C / UBCH10 Antibody  
(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

UBE2C / UBCH10 antibody
  Rabbit anti-UBE2C Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using UBE2C antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

UBE2C / UBCH10 (N-term) antibody
Western blot analysis of UBE2C (arrow) using rabbit polyclonal UBE2C Antibody (N-term G25) (AP17818PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2) (Origene Technologies).(8ug/ml) Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

UBE2C - human gene knockout kit via CRISPR
UBE2C - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2C - human gene knockout kit via CRISPR
UBE2C - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2C - human gene knockout kit via CRISPR
UBE2C - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2C - human gene knockout kit via CRISPR
UBE2C - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UBE2C / UBCH10 (transcript variant 4)
  Recombinant protein of human ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4  
UBE2C / UBCH10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

UBE2C / UBCH10 (transcript variant 1)
  Recombinant protein of human ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 1  
UBE2C / UBCH10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

UBE2C overexpression lysate
UBE2C overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

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  3. Rat (Rt) (2)
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Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (2)
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