UBE2E2 antibody
Principal name
UBE2E2 antibody
Alternative names for UBE2E2 antibody
Ubiquitin-conjugating enzyme E2 E2, Ubiquitin-protein ligase E2, Ubiquitin carrier protein E2, UBCH8
Ncbi ID
9606, NP_689866.1, NP_689866, NP_659088.1, NM_144839, NM_152653
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of UBE2E2 antibody
- Kit Component:
- - KN318571G1, Ube2e2 gRNA vector 1 in pCas-Guide vector
- KN318571G2, Ube2e2 gRNA vector 2 in pCas-Guide vector
- KN318571D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Plafker,S.M., (2004) J. Cell Biol. 167 (4), 649-659
Immunofluorescence of monoclonal antibody to UBE2E2 on HeLa cell . [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged UBE2E2 is approximately 0.03ng/ml as a capture antibody.
Human kidney; UBE2E2 antibody - N-terminal region (ARP43437_P050) in Human kidney cells using Immunohistochemistry
recombinant protein; . Lanes: 1: 40ng HIS-UBE2D1 protein. 2: 40ng HIS-UBE2D2 protein. 3: 40ng HIS-UBE2D3 protein. 4: 40ng HIS-UBE2D4 protein. 5: 40ng HIS-UBE2E1 protein. 6: 40ng HIS-UBE2E2 protein. 7: 40ng HIS-UBE2E3 protein. 8: 40ng HIS-UBE2K protein. 9: 40ng HIS-UBE2L3 protein. 10: 40ng HIS-UBE2N protein. 11: 40ng HIS-UBE2V1 protein. 12: 40ng HIS-UBE2V2 protein.. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:50,000. Gene Name: UBE2E2. Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK..; UBE2E2 antibody - N-terminal region (ARP43437_P050) in recombinant protein cells using Western Blot
Human Jurkat; WB Suggested Anti-UBE2E2 Antibody Titration: 0.125ug/ml. Positive Control: Jurkat cell lysate; UBE2E2 antibody - N-terminal region (ARP43437_P050) in Human Jurkat cells using Western Blot
Host: Rabbit; Target Name: Ube2e2; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0ug/ml
Lanes: 1: 40 ng HIS-UBE2D1 protein; 2: 40 ng HIS-UBE2D2 protein; 3: 40 ng HIS-UBE2D3 protein; 4: 40 ng HIS-UBE2D4 protein; 5: 40 ng HIS-UBE2E1 protein; 6: 40 ng HIS-UBE2E2 protein; 7: 40 ng HIS-UBE2E3 protein; 8: 40 ng HIS-UBE2K protein; 9: 40 ng HIS-UBE2
WB Suggested Anti-UBE2E2 Antibody Titration: 0.125ug/ml; Positive Control: Jurkat cell lysate
Human kidney
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304787 | UBE2E2 |
||||
| Recombinant protein of human ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) (UBE2E2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202054L2V | Lenti ORF particles, Ube2e2 (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) (Ube2e2), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202054L1V | Lenti ORF particles, Ube2e2 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) (Ube2e2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
