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VARS (Valyl-tRNA synthetase) antibody

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Principal name

VARS (Valyl-tRNA synthetase) antibody

Alternative names for VARS (Valyl-tRNA synthetase) antibody

VARS2, Valine-tRNA ligase, ValRS, G7A

SwissProt ID

P26640 (Human), Q04462 (Rat), Q9Z1Q9 (Mouse)

Gene ID

7407 (VARS), 22321 (Vars)

Ncbi ID

9606, NP_006286, AAH12808.1, NP_006286.1, NP_035820.3, NM_006295

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of VARS (Valyl-tRNA synthetase) antibody

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. VARS belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)

PAB3528 - VARS (Valyl-tRNA synthetase)
Western blot analysis of VARS polyclonal antibody ( Cat # PAB3528 ) in K-562 cell line lysates ( 35 µg/lane ) .VARS ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
PAB3529 - VARS (Valyl-tRNA synthetase)
Western blot analysis of VARS polyclonal antibody ( Cat # PAB3529 ) in HL-60 cell line lysates ( 35 µg/lane ) .VARS ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
AP14679PU-N - VARS (Valyl-tRNA synthetase)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the VARS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14679PU-N - VARS (Valyl-tRNA synthetase)
Western blot analysis of anti-VARS Antibody (N-term) in K562 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab (1:60dilution).
AP14680PU-N - VARS (Valyl-tRNA synthetase)
Western blot analysis of anti-VARS Antibody (Center) in HL60 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab (1:60dilution).
ARP46150_P050 - VARS (Valyl-tRNA synthetase)
Human 721_B; WB Suggested Anti-VARS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 721_B cell lysate. VARS is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; VARS antibody - middle region (ARP46150_P050) in Human 721_B cells using Western Blot
ARP46151_P050 - VARS (Valyl-tRNA synthetase)
Neurospora crassa; Data Provided by Dr. Margarida Duarte, IBMC PortugalThe antibody in Neurospora crassa mitochondria and post-mitochondrial supernatants.In this filamentous fungus a single gene encodes cytosolic and mitochondrial valyl tRNA synthetase.We used a 1:1000 dilution of the primary antibody (overnight incubation at 4ºC) followed by an HRP conjugated secondary antibody. Chemiluminescence detection was performed using the 'western lightning Ultra'from PerkinElmer.; VARS antibody - middle region (ARP46151_P050) in Neurospora crassa cells using Western Blot
ARP46151_P050 - VARS (Valyl-tRNA synthetase)
Human 721_B; WB Suggested Anti-VARS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. VARS is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; VARS antibody - middle region (ARP46151_P050) in Human 721_B cells using Western Blot
TP301089 - VARS (Valyl-tRNA synthetase)
TA339295 - VARS (Valyl-tRNA synthetase)
WB Suggested Anti-VARS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 721_B cell lysate.VARS is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
TA339296 - VARS (Valyl-tRNA synthetase)
Data Provided by Dr. Margarida Duarte, IBMC PortugalThe antibody in Neurospora crassa mitochondria and post-mitochondrial supernatants.In this filamentous fungus a single gene encodes cytosolic and mitochondrial valyl tRNA synthetase.We used a 1: 1000 dil
TA339296 - VARS (Valyl-tRNA synthetase)
WB Suggested Anti-VARS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate.VARS is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VARS (Valyl-tRNA synthetase) (N-term) antibody
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the VARS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

VARS (Valyl-tRNA synthetase) (Center) antibody
Western blot analysis of anti-VARS Antibody (Center) in HL60 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab (1:60dilution). Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VARS (Valyl-tRNA synthetase)
  Recombinant protein of human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein  
VARS (Valyl-tRNA synthetase) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.
 

3 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (2)
  2. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (2)
Target
  1. VARS (Valyl-tRNA synthetase) (3)
Property
  1. Center (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (2)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
Host
  1. Rabbit (2)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (3)
Service
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