WEE1 / Wee1-like protein kinase antibody
Principal name
WEE1 / Wee1-like protein kinase antibody
Alternative names for WEE1 / Wee1-like protein kinase antibody
Wee1A kinase, WEE1hu
Gene ID
7465 (WEE1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of WEE1 / Wee1-like protein kinase antibody
- Kit Component:
- - KN209760G1, WEE1 gRNA vector 1 in pCas-Guide vector
- KN209760G2, WEE1 gRNA vector 2 in pCas-Guide vector
- KN209760D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of Wee 1 expression in mouse small intestine tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with WEE1 (phospho S123) polyclonal antibody ( Cat # PAB0632 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma
WEE1 (phospho S123) polyclonal antibody ( Cat # PAB0632 ). Western blot analysis of WEE1 expression in human kidney.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with WEE1 polyclonal antibody ( Cat # PAB3321 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of WEE1 polyclonal antibody ( Cat # PAB3321 ) in HL-60 cell lysate. WEE1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with WEE1 polyclonal antibody ( Cat # PAB3322 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
The WEE1 polyclonal antibody ( Cat # PAB3322 ) is used in Western blot to detect WEE1 in Jurkat cell lysate ( Lane 1 ) and mouse liver tissue lysate ( Lane 2 ) .
WEE1 monoclonal antibody (M01A), clone 5B6 Western Blot analysis of WEE1 expression in Hela NE ( Cat # L013V3 ).
Proximity Ligation Analysis (PLA) of protein-protein interactions between WEE1 and FBXW11. HeLa cells were stained with anti-WEE1 rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of WEE1 expression in transfected 293T cell line (H00007465-T02) by WEE1 MaxPab polyclonal antibody.Lane 1: WEE1 transfected lysate(71.70 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Dot blot analysis of anti-Phospho-Wee1-Ser123 Antibody (Cat.#AP12735PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analysis of anti-WEE1 Pab in HL-60 cell lysate. WEE1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
The anti-WEE1 Pab is used in Western blot to detect WEE1 in Jurkat cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Western blot analysis of WEE1 expression in mouse small intestine tissue lysate.
Immunofluorescence of purified MaxPab antibody to WEE1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of WEE1 expression in transfected 293T cell line (H00007465-T01) by WEE1 MaxPab polyclonal antibody.Lane 1: WEE1 transfected lysate(71.06 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody AP55734PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from 293 cells, using WEE1 (Ab-53) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 60mins), using WEE1 (Phospho-Ser642) antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314672 | WEE1 / Wee1-like protein kinase antibody |
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| Rabbit polyclonal WEE1 (Ab-53) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA314673 | WEE1 / Wee1-like protein kinase antibody |
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| Rabbit polyclonal WEE1 (Ser642) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP12735PU-N | WEE1 / Wee1-like protein kinase pSer123 antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP15063PU-N | WEE1 / Wee1-like protein kinase (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP15064PU-N | WEE1 / Wee1-like protein kinase (Center) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP55734PU-N | WEE1 / Wee1-like protein kinase pSer642 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55734PU-S | WEE1 / Wee1-like protein kinase pSer642 antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309760 | WEE1 / Wee1-like protein kinase (transcript variant 1) |
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| Recombinant protein of human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
