LIMK2 antibody
Principal name
LIMK2 antibody
Alternative names for LIMK2 antibody
LIMK2, LIM domain kinase 2, LIMK-2, EC=2.7.11.1
Ncbi ID
NP_005560.1, 9606, NP_005560, NP_001026971.1, NM_001031801, NP_057952, NP_001029202.1, NM_024135, NM_005569, NM_016733
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of LIMK2 antibody
There are approximately 40 known eukaryotic LIM proteins, so med for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to D or R, the LIM motif probably mediates protein-protein interactions. LIM kise-1 and LIM kise-2 belong to a small subfamily with a unique combition of 2 N-termil LIM motifs and a C-termil protein kise domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Immunofluorescence of monoclonal antibody to LIMK2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of LIMK2 expression in transfected 293T cell line by LIMK2 monoclonal antibody (M01), clone 2H2-E11.Lane 1: LIMK2 transfected lysate(72.232 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged LIMK2 is approximately 0.3ng/ml as a capture antibody.
Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using LIMK2 (phospho-Thr505) antibody (#AP02415PU).
Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using LIMK2 (phospho-Thr505) antibody (#AP02415PU, Red).
Figure 2. Western blot analysis of extract from NIH/3T3 cells treated or untreated with UV (60 min), using LIMK2 (phospho-Thr505) antibody (AP02415PU).
Western blot (WB) analysis of LIMK2 antibody (Cat.-No.: AP06211PU-N) in extracts from NIH/3T3 treated with UV.
Immunofluorescence of purified MaxPab antibody to LIMK2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of LIMK2 expression in transfected 293T cell line (H00003985-T01) by LIMK2 MaxPab polyclonal antibody.Lane1:LIMK2 transfected lysate(75.46 KDa).Lane2:Non-transfected lysate.
Immunohistochemistry (IHC) analysis of LIMK2 pThr505 antibody (Cat.-No.: AP01629PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of LIMK2 pThr505 antibody (Cat.-No.: AP01629PU-N) in extracts from COLO cells.
LIMK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of LIMK2 expression in mouse kidney.
LIMK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of LIMK2 expression in human liver.
Western Blot analysis of LIMK2 expression in transfected 293T cell line (H00003985-T01) by LIMK2 MaxPab polyclonal antibody.Lane 1: LIMK2 transfected lysate(77.90 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry: AP20423PU-N LIMK2 antibody staining of Paraffin-Embedded Human brain tissue.
Western blot (WB) analysis of AP20423PU-N LIMK2 antibody in extracts from HepG2 cells.
Immunohistochemical analysis of LIMK2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of LIMK2 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of LIMK2 expression in Jurkat (A); COLO205 (B); MCF7 (C) whole cell lysates.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LIMK2 rabbit polyclonal antibody. (TA590208)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LIMK2 rabbit polyclonal antibody. (TA590208)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-LIMK2 rabbit polyclonal antibody. (TA590208)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LIMK2 rabbit polyclonal antibody. (TA590208)
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK2 (RC217705, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK2.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: LIM kinase 2 Antibody
Sample (30ug whole cell lysate). A:293T. 7.5% SDS PAGE. TA308918 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded CA922 xenograft , using LIM kinase 2(TA308918) antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using LIM kinase 2(TA308918) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 7.5% SDS PAGE. TA308919 diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded Breast ca, using LIM kinase 2(TA308919) antibody at 1:250 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A549, using LIM kinase 2(TA308919) antibody at 1:200 dilution.
Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using LIMK2 (Ab-283) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2) and cos-7 cells (Lane 3), using LIMK2 (Ab-283) Antibody. The lane on the left is treated with synthesized peptide.
Western blot analysis of extracts from HepG2 cells and HUVEC cells, using LIMK2 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human heart tissue using LIMK2 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using LIMK2 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of LIMK2 expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using LIMK2 antibody.
Western blot analysis of extracts from Hela cells, using Phospho-LIMK2-Thr505 antibody.
Western blot analysis of LIMK2 phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using LIMK2 (Phospho-Ser283) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from NIH/3T3 cells treated and untreated with UV (60min), using LIMK1 (phospho-Thr505) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (phospho-Thr505) antibody.
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA590208 | LIMK2 antibody |
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| Rabbit Polyclonal LIM kinase 2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
0.1 mg /
€409.00
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| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody! |
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| TA313233 | LIMK2 antibody |
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| Rabbit polyclonal LIMK2 (Ab-283) antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA313235 | LIMK2 antibody |
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| Rabbit polyclonal LIMK2 (Ser283) antibody(Phospho-specific) | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||