MAP-2 antibody

Background of MAP-2 antibody

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alterte transcription, and all share a C-termil sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neurol cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neurol cells, their perikarya and neurol dendrites. In contrast tau is found predomintly in neurol axons.