MAP2K5 antibody
Principal name
MAP2K5 antibody
Alternative names for MAP2K5 antibody
MAP kinase kinase 5, MAPKK 5, MEK5, MKK5, Dual specificity mitogen-activated protein kinase kinase 5
Ncbi ID
NP_001193733.1, 9606, 10116, NP_001029159.1, NP_002748, NM_011840, NP_660143.1, NP_035970.1, BC028260, NM_001033987, NM_017246, NM_145160, NM_002757
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot), Immunoprecipitation (IP)
Background of MAP2K5 antibody
- Kit Component:
- - KN309723G1, Map2k5 gRNA vector 1 in pCas-Guide vector
- KN309723G2, Map2k5 gRNA vector 2 in pCas-Guide vector
- KN309723D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection of endogenous MKK5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK5-14B5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Immunofluorescence of monoclonal antibody to MAP2K5 on HeLa cell . [antibody concentration 10 ug/ml]
MAP2K5 monoclonal antibody (M01), clone M1-E6. Western Blot analysis of MAP2K5 expression in K-562 ( Cat # L009V1 ).
MAP2K5 monoclonal antibody (M01), clone M1-E6 Western Blot analysis of MAP2K5 expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged MAP2K5 is approximately 0.03ng/ml as a capture antibody.
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K3 and MAP2K5 HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-MAP2K5 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Detection limit for recombinant GST tagged MAP2K5 is approximately 10ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to MAP2K5 on HeLa cell. [antibody concentration 10 ug/ml]
MAP2K5 monoclonal antibody (M11), clone 3E10 Western Blot analysis of MAP2K5 expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged MAP2K5 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to MAP2K5 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of MAP2K5 expression in transfected 293T cell line (H00005607-T01) by MAP2K5 MaxPab polyclonal antibody.Lane 1: MAP2K5 transfected lysate(49.28 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 polyclonal antibody ( Cat # PAB2270 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of MAP2K5 ( arrow ) using rabbit MAP2K5 polyclonal antibody ( Cat # PAB2270 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the MAP2K5 gene ( Lane 2 ) ( Origene Technologies ) .
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of MAP2K5 (arrow) using rabbit polyclonal MAP2K5 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2)
The whole cell lysates derived from EGF-stimulated A-431 were immunoblotted by MAP2K5 ( phospho S311/T315 ) polyclonal antibody ( Cat # PAB12634 ) at 1 : 1000 ( lane 1 ). The lane 2 was pre-absorbed by immunizing peptide.
Dot Blot : 1 µg peptide was blot onto NC membrane.A : MAP2K5( pS311/pT315 ).B : MAP2K5 ( Non-phospho ).C : Non-related phospho-specific.Followed were blotted at a 1 : 1000 dilution by :1. MAP2K5 ( phospho S311/T315 ) polyclonal antibody ( Cat # PAB12634 ).2 : MAP2K5 polyclonal antibody ( Cat # PAB12705 ).
The tissue lysates derived from mouse liver were immunoblotted by MAP2K5 polyclonal antibody ( Cat # PAB12704 ) at 1 : 1000.
The tissue lysates derived from mouse liver were immunoblotted by MAP2K5 polyclonal antibody ( Cat # PAB12705 ) at 1 : 1000 ( lane 1 ). The lane 2 was a negative control.
Dot Blot : 1 µg peptide was blot onto NC membrane.A : MAP2K5 ( pS311/pT315 ).B : MAP2K5 ( Non-phospho ).C : Non-related phospho-specific phosphopeptide.Followed were blotted at a 1 : 1000 dilution by :1. Phospho-MAP2K5 S311/T315 polyclonal antibody ( Cat # PAB12634 ).2. MAP2K5 polyclonal antibody ( Cat # PAB12705 ).
Western blot of rat testis lysate showing specific labeling of the ~49k Map2k5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
Figure 1. Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
Immunoprecipitation of MAP2K5 transfected lysate using anti-MAP2K5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MAP2K5 MaxPab mouse polyclonal antibody (B01) (H00005607-B01).
Mouse Heart; WB Suggested Anti-Map2k5 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Map2k5 antibody - N-terminal region (ARP49021_P050) in Mouse Heart cells using Western Blot
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using MAP2K5 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using MAP2K5 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of MKK5 expression in Hela (A); NIH3T3 (B); PC12 (C) whole cell lysates.
Immunohistochemical analysis of MKK5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of extracts from Rat and Mouse brain tissue using phospho-Map2k5-S311/T315 antibody.
Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP05673PU-N | MAP2K5 pSer311/pThr315 antibody |
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Rabbit | IgG | Purified | Bov, Can, Chk, Hu, Mky, Ms, Rt, Xen, Ze | WB |
0.1 ml /
€590.00
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| OriGene Technologies GmbH | |||||||
