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MELK antibody

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Principal name

MELK antibody

Alternative names for MELK antibody

Maternal embryonic leucine zipper kinase, KIAA0175, hMELK

SwissProt ID

Q14680 (Human), Q61846 (Mouse)

Gene ID

9833 (MELK), 17279 (Melk)

Ncbi ID

NP_055606.1, 9606, NP_055606, NM_014791.2, NM_014791, NP_034920.2, NM_010790

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of MELK antibody

Melk is a Serine/threonine-protein kise involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Melk Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It Plays a key role in cell proliferation. It is required for proliferation of embryonic and posttal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. It's also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus and may also play a role in primitive hematopoiesis.

General readings

1. Lin ML et al. Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family. Breast Cancer Res. 2007;9(1):R17.
PMID: 17280616

PAB2705 - MELK
The MELK polyclonal antibody ( Cat # PAB2705 ) is used in Western blot to detect MRLK in mouse brain tissue lysate.
PAB6013 - MELK
MELK polyclonal antibody ( Cat # PAB6013 ) staining ( 0.1 µg/ml ) of human testes lysate ( RIPA buffer, 1.4E5 cells per lane ) . Detected by western blot using chemiluminescence.
AP13789PU-N - MELK
The anti-MRLK Pab is used in Western blot to detect MRLK in mouse brain tissue lysate
AP13790PU-N - MELK
The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate
ARP46207_P050 - MELK
Human HT1080; WB Suggested Anti-MELK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate; MELK antibody - middle region (ARP46207_P050) in Human HT1080 cells using Western Blot
AP20443PU-N - MELK
Immunohistochemistry (IHC) analyzes of MELK antibody (Cat.-No.: AP20443PU-N) in paraffin-embedded human liver carcinoma tissue.
AP20443PU-N - MELK
Western blot (WB) analysis of MELK antibody (Cat.-No.: AP20443PU-N) in extracts from k562 cells.
C11043-1 - MELK
Western blot analysis of extracts from K562 cells, using MELK Antibody. The lane on the right is treated with the synthesized peptide.
AM06778PU-N - MELK
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.
AM06778PU-N - MELK
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MELK mouse mAb with DAB staining.
AM06778PU-N - MELK
Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06778PU-N - MELK
Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);
AM06778PU-N - MELK
Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).
CPA3373-100ul - MELK
Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3373-100ul - MELK
Immunofluorescent analysis of MELK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA3373-100ul - MELK
Western blot analysis of MELK expression in MCF7 (A); NIH3T3 (B); mouse liver (C); rat liver (D) whole cell lysates.
TA302675 - MELK
TA302675 (0.1µg/ml) staining of human testes lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
TA339317 - MELK
WB Suggested Anti-MELK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysate
TA339318 - MELK
Host: Rabbit; Target Name: Melk; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MELK (C-term) antibody
  Goat Polyclonal Antibody against MELK  
TA302675 (0.1µg/ml) staining of human testes lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

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  1. Primary Antibody (1)
Target
  1. MELK (1)
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  1. C-term (1)
Applications
  1. Enzyme Immunoassay (E) (1)
  2. Western blot / Immunoblot (WB) (1)
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  1. Aff - Purified (1)
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  1. Goat (1)
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  1. 1 image (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (1)
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  1. OriGene Technologies (1)
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