TA319879 HIGD1A (N-term) antibody

Rabbit Polyclonal HIG1 Antibody

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0.1 mg / €415.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human HIGD1A

TA319879

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Product Description for HIGD1A

Rabbit anti Human HIGD1A.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for HIGD1A

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms HIG1, HIG1 domain family member 1A mitochondrial, Hypoxia-inducible gene 1 protein, RCF1 homolog A, RCF1a
Presentation Aff - Purified
Reactivity Hu
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y241
Immunogen:
19 amino acid synthetic peptide near the amino terminus of Human HIG1 (AP55555CP-N)
GeneID:
25994
Property N-term
Add. information Blocking peptide available: AP55555CP-N
Application Western blot: 0.5-1 μg/ml.
Positive Control: 293 cell lysate.
Immunofluorescence: Start at 10 μg/ml.
Immunocytochemistry: Start at 2.5 μg/ml.
Background HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG1 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.
Concentration 1.0 mg/ml
General Readings
  1. Bedo G, Vargas M, Ferreiro MJ, Chalar C, Agrati D. Characterization of hypoxia induced gene 1: expression during rat central nervous system maturation and evidence of antisense RNA expression. Int J Dev Biol. 2005;49(4):431-6. PubMed PMID: 15968589.
  2. Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S. Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 2000 Sep;1(3):287-92. PubMed PMID: 11256614. (Free PMC Article available, 2 images available)
  3. Denko N, Schindler C, Koong A, Laderoute K, Green C, Giaccia A. Epigenetic regulation of gene expression in cervical cancer cells by the tumor microenvironment. Clin Cancer Res. 2000 Feb;6(2):480-7. PubMed PMID: 10690527.
  4. Gimm T, Wiese M, Teschemacher B, Deggerich A, Schödel J, Knaup KX, et al. Hypoxia-inducible protein 2 is a novel lipid droplet protein and a specific target gene of hypoxia-inducible factor-1. FASEB J. 2010 Nov;24(11):4443-58. doi: 10.1096/fj.10-159806. Epub 2010 Jul 12. PubMed PMID: 20624928.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% Sodium Azide as preservative
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is predicted not to cross-react with HIG2

Accessory Products

Proteins and/or Positive Controls

Positive controls for HIGD1A (1 products)

Catalog No. Species Pres. Purity   Source  

HIGD1A Lysate

Western Blot: HIGD1A Lysate [NBL1-11542] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for HIGD1A.
  Novus Biologicals Inc.
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