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AP31948PU-N PDE4B (C-term) antibody

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0.1 mg / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

View Datasheet

OriGene recommends this product for Western blotting.

Quick Overview

Goat anti Human, Mouse PDE4B

AP31948PU-N

Product Description for PDE4B

Goat anti Human, Mouse PDE4B.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for PDE4B

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms 5'-cyclic phosphodiesterase 4B, DPDE4, EC=3.1.4.17, PDE32, PDE4B, cAMP-specific 3'
Presentation Aff - Purified
Reactivity Hu, Ms
Applications E, WB
Clonality Polyclonal
Host Goat
Molecular weight 83.3 kDa (NP_002591.2).
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q07343
Immunogen:
syntehtic peptide (C-IDIATEDKSPVDT) from the C Terminus of the protein sequence according to NP_002591.2; NP_001032416.1; NP_001032417.1.
GeneID:
5142
Property C-term
Application
Peptide ELISA: 1/32000 (Detection limit). 
Western blot: 1-3 µg/ml. Approx 98kDa band observed in Human Uterus lysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Méhats et al, Endocrinology. 1999 Jul;140(7):3228-37. PMID: 10385419).
Background Cyclic nucleotide phosphodiesterases (PDEs) catalyse the hydrolytic inactivation of the common intracellular second messengers cyclic adenosine and guanosine 3', 5'-monophosphate (cAMP and cGMP). Thus, these enzymes play a critical role in the regulation of a wide range of physiological processes modulated by cyclic nucleotide signalling. The PDE4 enzyme belongs to a family of cAMP-dependent PDEs that provide the major means of inactivating the key intracellular second messenger, cAMP.Four genes (4A, 4B, 4C, and 4D) encode around 20 distinct isoform members of the PDE4 family. Each isoform is characterized by a unique N-terminal region.
Concentration 0.5 mg/ml
Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. 
Shelf life: one year from despatch. 
Format
Purification:
Affinity Chromatography
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is expected to recognize all reported isoforms (NP_002591.2, NP_001032416.1, NP_001032417.1).
Gene ID 5142

Accessory Products

Proteins and/or Positive Controls

Proteins for PDE4B (4 products)

Catalog No. Species Pres. Purity   Source  

PDE4B (transcript variant d)
PDE4B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

PDE4B (transcript variant a)
PDE4B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

PDE4B
PDE4B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PDE4B
PDE4B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for PDE4B (3 products)

Catalog No. Species Pres. Purity   Source  

PDE4B Lysate
Western Blot: PDE4B Lysate [NBL1-14219] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PDE4B.
  Novus Biologicals Inc.

PDE4B Lysate
Western Blot: PDE4B Lysate [NBL1-14220] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PDE4B.
  Novus Biologicals Inc.

PDE4B overexpression lysate
PDE4B overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

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Goat IgG (H+L chain) antibody
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Goat IgG (H+L chain) antibody
Goat IgG (H+L chain) antibody Rabbit TRITC E, ICC/IF
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Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Goat IgG (H+L chain), adsorbed antibody
Goat IgG (H+L chain), adsorbed antibody Donkey Cy2
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Goat IgG (H+L chain), adsorbed antibody
Goat IgG (H+L chain), adsorbed antibody Donkey Cy3
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Goat IgG (H+L chain), adsorbed antibody
Goat IgG (H+L chain), adsorbed antibody Donkey Cy5 F, ICC/IF
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Click here to see all secondary antibodies for PDE4B
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