Properties for 5X Antigen-Down Coating Buffer 1 (CB2)
| Product Category | Buffers & Chromogens |
|---|---|
| Quantity | 100 ml |
| Synonyms | Coating buffer, ELISA coating buffer, ELISA diluents |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Add. information | Add 1 part CB2 to 4 parts deionized water and mix for 5-15 minutes. Add the antigen to be coated to the 1X buffer and mix for 5-15 minutes. Coat the plate by pipetting the solution into the wells of the ELISA plate. |
|---|---|
| Application | ELISA, Add 1 part CB2 to 4 parts deionized water and mix for 5-15 minutes. Add the antigen to be coated to the 1X buffer and mix for 5-15 minutes. Coat the plate by pipetting the solution into the wells of the ELISA plate., ELISA , |
| Background | 5X Antigen-Down Plate Coating Buffer (CB2) maximizes the adsorption of antigens onto polystyrene plates for use in Antigen-down ELISA formats. During the adsorption process, CB2 stabilizes coated proteins by maintaining their three-dimensional structure. By stabilizing the adsorbed protein, antigenic regions are preserved, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents. - Stabilizes the tertiary three-dimensional structure of antigens to enhance their binding reactivity with antibody. - Buffered reagent to coat antigens onto polystyrene microtiter ELISA plates. - Enhances adsorption of antigens onto polystyrene microtiter ELISA plates. - pH 8.4 at 1X, ionic strength. - Plates can be coated at room temperature. - Retards bacterial growth. - Can be used at 50uL - 300uL per well at 1X. |
| Storage | Store at 4C. Do not freeze. |
| Specificity |
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