discontinued

AP05629PU-N Synapsin-1 pSer549 antibody

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Rabbit anti Bovine, Canine, Human, Monkey, Mouse, Rat Synapsin-1

AP05629PU-N

Product Description for Synapsin-1

Rabbit anti Bovine, Canine, Human, Monkey, Mouse, Rat Synapsin-1.
Properties: pSer549
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Synapsin-1

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Brain protein 4.1, SYN1, Synapsin I
Presentation Purified
Reactivity Bov, Can, Hu, Mky, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17600
Immunogen:
Phosphopeptide corresponding to amino acid sequence surrounding the phosphorylated Serine 549 of Synapsin I.
GeneID:
6853
Property pSer549
Application Western Blot: 1:/000. Detects a band of approximately 78kDa in Rat cortex cell lysates. 
Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Background Synapsin I is a 78 kDa member of the synapsin family, expressed in the central and peripheral nervous system. Synapsins coat synaptic vesicles, binding them to the cytoskeleton and are thought to regulate their availability for release. Synapsins are important in brain plasticity, axonogenesis and synaptogenesis. Mutations in the synapsin I gene may be associated with disorders such as X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders.
General Readings
  1. Baldelli P, Fassio A, Corradi A, Cremona O, Valtorta F, Benfenati F. Synapsins and neuroexocytosis: recent views from functional studies on synapsin null mutant mice. Arch Ital Biol. 2005 May;143(2):113-26. PubMed PMID: 16106992.
  2. Garcia CC, Blair HJ, Seager M, Coulthard A, Tennant S, Buddles M, et al. Identification of a mutation in synapsin I, a synaptic vesicle protein, in a family with epilepsy. J Med Genet. 2004 Mar;41(3):183-6. PubMed PMID: 14985377. (Free PMC Article available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
10mM HEPES, pH7.5
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.01% BSA, 50% Glycerol
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (expected):
Bovine, Canine, Human, Mouse, Primate.
Species reactivity (tested):
Rat.
Specificity
Specificity:
This antibody recognises synapsin I, phosphorylated at Serine residue 549.
Phosphorylation of Serine residue 549 by MAP kinase or cyclin-dependent kinases 1 or 5 is an important part in modulating synaptic vesicle trafficking.
Gene ID 6853

Accessory Products

Proteins and/or Positive Controls

Proteins for Synapsin-1 (3 products)

Catalog No. Species Pres. Purity   Source  

Synapsin-1 (transcript variant Ia)

Synapsin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Synapsin-1

Synapsin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Synapsin-1

Synapsin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Positive controls for Synapsin-1 (2 products)

Catalog No. Species Pres. Purity   Source  

SYN1 overexpression lysate

SYN1 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

SYN1 overexpression lysate

SYN1 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.
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