AR05010PU-N ACE / CD143

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0.25 U / €390.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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ACE / CD143

AR05010PU-N

Product Description for ACE / CD143

ACE / CD143.
Presentation: Purified

Properties for ACE / CD143

Product Category Proteins & Growth Factors
Quantity 0.25 U
Synonyms Angiotensin-converting enzyme, DCP, DCP1, Dipeptidyl carboxypeptidase I
Presentation Purified
Source Lung
Species (Protein) Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P12822
Application Functional Assays.
Background ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.
Concentration 10 Units/ml.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Source Rabbit
Description
Description:
Purified ACE from Rabbit lung.
The Km for this enzyme is 2.6 mM using hippuryl-L-histidyl-L-leucine as the substrate.
Biological activity:
One unit will produce 1.0 micromole of hippuric acid from hippuryl-His-Leu per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37ºC.
Inactivation:
EDTA can be used as a strong inhibitor of this enzyme. This can be reversed by dialysis and reactivation with metal salts.
Molecular weight:
Reported as approximately 129 kDa (sedimentation equilibrium with and without 6 M guanidine hydrochloride) and approximately 140 kDa (SDS gel electrophoresis and by gel filtration)
Specific activity:
4.5 U/mg protein.
Format
Preservatives:
None
Stabilizers:
None
State:
Liquid purified protein from Rabbit lung.
Purified
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