discontinued

AM05574PU-N CD279 / PD1 antibody

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Quick Overview

Rat anti Mouse CD279 / PD1 RMP1-14

AM05574PU-N

Product Description for CD279 / PD1

Rat anti Mouse CD279 / PD1 RMP1-14.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry.

Properties for CD279 / PD1

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1
Presentation Purified
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone RMP1-14
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q02242
Immunogen:
PD-1 transfected BHK cells
GeneID:
18566
Isotype control SM15P, SM15PX
Application Immunohistochemistry on frozen sections.
Flow Cytometry: 1/10 - 1/25.
Background CD279 is expressed predominantly on activated T- and B- lymphocytes and on a subset of thymocytes.
Studies suggest that CD279, an immunoinhibitory receptor, plays a critical role in peripheral tolerance induction and prevention of autoimmune disease. Two members of the B7 family, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified as the ligands for CD279.
Caution (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Concentration 1.0 mg/ml
General Readings
  1. Yamazaki T, Akiba H, Koyanagi A, Azuma M, Yagita H, Okumura K. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 2005 Aug 1;175(3):1586-92. PubMed PMID: 16034097.
  2. Matsumoto K, Inoue H, Nakano T, Tsuda M, Yoshiura Y, Fukuyama S, et al. B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism. J Immunol. 2004 Feb 15;172(4):2530-41. PubMed PMID: 14764726.
  3. Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, et al. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003 Oct 15;171(8):4156-63. PubMed PMID: 14530338.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein G
Buffer System:
Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide
Purity:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
State:
Liquid purified IgG
Purified
Specificity
Specificity:
This antibody recognises CD279, a 55kD cell surface protein which is a member of the CD28/CTLA-4 family, otherwise known as programmed death-1 (PD-1). Antibodies produced by clone RMP1-14 have been reported to block the binding of both B7-H1-Ig and B7-DC-Ig fusion proteins to PD-1 transfected BHK cells.
Species:
Mouse.
Gene ID 18566

Accessory Products

Proteins and/or Positive Controls

Proteins for CD279 / PD1 (2 products)

Catalog No. Species Pres. Purity   Source  

CD279 / PD1 (C-term DDK/His tag)

CD279 / PD1 Mouse > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD279 / PD1 (C-term Fc tag)

CD279 / PD1 Mouse > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
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