Product Description for Ly6A/E / SCA1
Rat anti Mouse Ly6A/E / SCA1 D7.
Presentation: Biotin
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.
Presentation: Biotin
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.
Properties for Ly6A/E / SCA1
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP |
| Presentation | Biotin |
| Reactivity | Ms |
| Applications | C, F, IP, WB |
| Clonality | Monoclonal |
| Clone | D7 |
| Host | Rat |
| Isotype | IgG2a |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Western Blot. Immunoprecipitation. Flow Cytometry: 1/5 - 1/10. Use 10 µl of suggested working dilution to label 1 x 10^6 cells in 100 µl. Immunohistochemistry on frozen sections. |
| Background | Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells. |
| Concentration | 0.5 mg/ml |
| General Readings |
|
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 110454 |
Accessory Products
Recommended Secondary Antibodies for Ly6A/E / SCA1 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1371AP |
Rat IgG (H+L chain) antibody |
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|
Rabbit | AP | |||||
| OriGene Technologies GmbH | |||||||
| R1371B |
Rat IgG (H+L chain) antibody |
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|
|
Rabbit | Biotin | |||||
| OriGene Technologies GmbH | |||||||
| R1371F |
Rat IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| OriGene Technologies GmbH | |||||||
| R1371HRP |
Rat IgG (H+L chain) antibody |
||||||
|
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1371T |
Rat IgG (H+L chain) antibody |
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|
|
Rabbit | TRITC | |||||
| OriGene Technologies GmbH | |||||||
| R1413R |
Rat IgG (H+L chain), F(ab)2 Fragment antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1415R |
Rat IgG F(ab)2 specific (adsorbed F(ab)2 Fragment) antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1428R |
Rat IgG (H+L chain) F(ab')2 Fragment antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1439C2 |
Rat IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1439C3 |
Rat IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1439C5 |
Rat IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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