AP08132PU-N Human Kappa Light Chain antibody

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1 ml / €320.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Monkey Human Kappa Light Chain

AP08132PU-N

Product Description for Human Kappa Light Chain

Rabbit anti Human, Monkey Human Kappa Light Chain.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Human Kappa Light Chain

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Hu, Mky
Applications C, ICC/IF, P
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified human Kappa light chain from human Bence Jones proteins.
Remarks:
Molecular weight of antigen: 25 kDa
Application Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system.
FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes).
Recommended positive control: Human tonsil.
Background Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin's (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30; most of them are bound to heavy chains of immunoglobulin.
Concentration 2.0 mg/ml
General Readings
  1. Korkolopoulou, P et.al. Leukemia and Lymphoma, 13: 151-159, 1994.
  2. Schmid, C. et.al. Am. J. Pathology 94: 1281-1289, 1991.
  3. Faure GC et.al. Am. J. Clin. Pathology 94: 571-575, 1990.
  4. Picken MM et. al. Arthritis and Rheumatism. 33: 880-884, 1990
  5. Faa G et. al. J of Hepatology. 12: 75-82, 1991.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Buffer System:
PBS, pH 7.4 containing 0.05% Sodium Azide as preservative and 1% BSA as stabilizer.
State:
Liquid purified Ig fraction.
Purified
Specificity
Specificity:
This antibody is useful in identification of leukemias, plasmasacytomas and certain non-Hodgkin's lymphomas. In normal human serum lambda chains are present 1.6-15.2 mg/ml. In multiple myeloma or B-cell malignancies these levels are increased. Higher amounts are also present in Bence Jones patient's urine.
Cellular Localization: Cytoplasmic.
Species:
Human and Monkey.

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