AR09147PU-N Endoplasmin / HSP90B1 / TRA1 (22-803, His-tag)

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0.1 mg / €570.00
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Endoplasmin / HSP90B1 / TRA1

Product Description for Endoplasmin / HSP90B1 / TRA1

Endoplasmin / HSP90B1 / TRA1.
Presentation: Purified

Properties for Endoplasmin / HSP90B1 / TRA1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1
Presentation Purified
Molecular weight 94.4 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14625
Purity > 90 % by SDS - PAGE
Background GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Randow F., et al. (2001). Nat Cell Biol. 3(10):E231.
Li Z., et al. (2002). Front Biosci. 7:d731-51.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human GRP94 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMDDE VDVDGTVEED LGKSREGSRT DDEVVQREEE AIQLDGLNAS QIRELREKSE KFAFQAEVNR MMKLIINSLY KNKEIFLREL ISNASDALDK IRLISLTDEN ALSGNEELTV KIKCDKEKNL LHVTDTGVGM TREELVKNLG TIAKSGTSEF LNKMTEAQED GQSTSELIGQ FGVGFYSAFL VADKVIVTSK HNNDTQHIWE SDSNEFSVIA DPRGNTLGRG TTITLVLKEE ASDYLELDTI KNLVKKYSQF INFPIYVWSS KTETVEEPME EEEAAKEEKE ESDDEAAVEE EEEEKKPKTK KVEKTVWDWE LMNDIKPIWQ RPSKEVEEDE YKAFYKSFSK ESDDPMAYIH FTAEGEVTFK SILFVPTSAP RGLFDEYGSK KSDYIKLYVR RVFITDDFHD MMPKYLNFVK GVVDSDDLPL NVSRETLQQH KLLKVIRKKL VRKTLDMIKK IADDKYNDTF WKEFGTNIKL GVIEDHSNRT RLAKLLRFQS SHHPTDITSL DQYVERMKEK QDKIYFMAGS SRKEAESSPF VERLLKKGYE VIYLTEPVDE YCIQALPEFD GKRFQNVAKE GVKFDESEKT KESREAVEKE FEPLLNWMKD KALKDKIEKA VVSQRLTESP CALVASQYGW SGNMERIMKA QAYQTGKDIS TNYYASQKKT FEINPRHPLI RDMLRRIKED EDDKTVLDLA VVLFETATLR SGYLLPDTKA YGDRIERMLR LSLNIDPDAK VEEEPEEEPE ETAEDTTEDT EQDEDEEMDV GTDEEEETAK ESTAEKDEL
Molecular weight:
(819 aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 0.1 M NaCl, 10% glycerol
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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