Product Description for FKBP1A / FKBP12
FKBP1A / FKBP12.
Presentation: Purified
Presentation: Purified
Properties for FKBP1A / FKBP12
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | FK506-binding protein 1A, Immunophilin FKBP12, PPIase FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, Rotamase |
| Presentation | Purified |
| Molecular weight | 14.1 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 95 % by SDS - PAGE |
| Background | FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2.Add 10 ul of recombinant FKBP1a/FKBP12 protein with 1 ug in assay buffer. 3.Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4.Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5.Record the increase in A405 nm for 30 minutes at 25°C. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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