DA3564 Bovine ECGFpro2 (complete for Lymphatic ECs)

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Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description:

Bovine Endothelial Cell Growth Factor (ECGFpro2) (For cultivation of lymphatic endothelial cells, LEC). 
ECGFpro2 is supplemented with recombinant human VEGF-C (corresponding to 50 ng/ml) a concentration sufficient for the cultivation of lymphatic endothelial cells. 
The ECGF + VEGF-C are sufficient for 500 ml growth medium.
Species specificity: Bovine ECGFpro2 is effective on Mouse, Bovine and Human cells.

Buffer System:

H₂O

Preservatives:

None

State:

Lyophilized, Freeze dried  powder (Crude extract)

Reconstitution:

Endothelial cell growth factor is supplied as a sterile lyophilized powder containing 6 mg protein per vial. To obtain a stock solution reconstitute the contents of the vial in 2 ml of prewarmed (37°C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. Although the stock solution can be added aseptically to sterile tissue culture medium, it is recommended that medium containing diluted product is aseptically filtered prior to use.
The ECGF + VEGF-C are sufficient for 500 ml growth medium.