Profilin-1 / PFN1 (1-140) - AR09259PU-L

Product Description for Profilin-1 / PFN1

Profilin-1 / PFN1.
Presentation: Purified

Immunogen	Swiss Prot Num: P07737
Purity	> 95 % by SDS - PAGE
Background	Profilin 1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Kwiatkowski DJ, et al. (1988) J. Biol. Chem. 263(12): 5910-5. Harbeck B., et al. (2000) J. Biol. Chem. 275(40): 30817-25.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch
Description	Description: Recombinant Profilin1 protein was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY` Molecular weight: (140 aa)
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol Endotoxin level: < 1.0 EU per 1 µg of protein (determined by LAL method ) Purity: >95% by SDS - PAGE State: Liquid purified protein Purified