AR09284PU-L GST-Tag (1-224, His-tag)

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0.5 mg / €720.00
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GST-Tag

Product Description for GST-Tag

GST-Tag.
Presentation: Purified

Properties for GST-Tag

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms GST26-Tag, Glutathione S-transferase Tag
Presentation Purified
Molecular weight 28.3 kDa
Source E. coli
Species (Protein) Schistosoma
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P08515
Purity > 95 % by SDS - PAGE
Background Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin.
Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source.
Concentration 1.0 mg/ml (determined by Bradford assay)
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant GST, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
Biological activity:
2.8-3.3 units/mg, defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
Activity Assay
1.Prepare a 1 ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol.
2.Equilibrate to 25°C and monitor at A340nm until the value is constant using a spectrophotometer.
3.Add 50 ul of recombinant GST protein with various concentrations (1ug, 2ug, 5ug) in 950 ul reaction buffer.
4.Mix by inversion and record the increase at A340nm for 5 minutes.
Molecular weight:
(244 aa), confirmed by MALDI-TOF
Format
Buffer System:
PBS, pH 7.4, 10% glycerol
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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