AKT1 / PKB (Substrate) - AR08011PU-N

Product Description for AKT1 / PKB

AKT1 / PKB.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Presentation	Purified
Molecular weight	817 Da
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P31749
Purity	> 98 % pure by HPLC analysis.
Application	Western Blot: 1/500-1/2,000.
Background	Akt is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when activated. Akt associates with PI(3,4,5)P3 at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.
Concentration	1.0 mg/ml (by weight)
General Readings	Alessi DR, Caudwell FB, Andjelkovic M, Hemmings BA, Cohen P. Molecular basis for the substrate specificity of protein kinase B; comparison with MAPKAP kinase-1 and p70 S6 kinase. FEBS Lett. 1996 Dec 16;399(3):333-8. PubMed PMID: 8985174.
Storage	Store the antibody (ibn aliquots) at -70°C or below prior to opening. Avoid repeated freezing and thawing. Shelf life: 6 month from despatch.
Description	Description: A specific substrate for Protein Kinase B alpha (PKB alpha, AKT; Km = 5 µM). This peptide is not phosphorylated by p70 S6 kinase or MAP kinase activated protein (MAPKAP) kinase-1.
Format	Purity: >98% pure by HPLC analysis. State: Liquid sterile filtered peptide Purified