Product Description for Cytokeratin 7
Mouse anti Bovine, Human Cytokeratin 7 C-46.
Properties: (+ KRT17)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties: (+ KRT17)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties for Cytokeratin 7
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin |
| Presentation | Purified |
| Reactivity | Bov, Hu |
| Applications | C, ICC/IF, IP, P, WB |
| Clonality | Monoclonal |
| Clone | C-46 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | + KRT17 |
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | Immunoprecipitation. Immunocytochemistry. Western Blot: 1 µg/ml Sample Preparation: Lysis 1% lauryl maltoside fo for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.) Positive Control: HeLa Human cell line. Immunohistochemistry on Paraffin Sections: 20 µg/ml. Pretreatment: 0.1% pepsin, 30 min, 37°C. Incubation: 4°C overnight; Chromogen DAB. Positive tissue: Pancreas. |
| Background | Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. |
| Concentration | 1.0 mg/ml |
| Product Citations |
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| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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| Specificity |
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| Gene ID | 3855 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cytokeratin 7 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00003855-P01-10 |
Cytokeratin 7 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003855-P01-25 |
Cytokeratin 7 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Cytokeratin 7 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-12396 |
Cytokeratin 7 Lysate |
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| Novus Biologicals Inc. | |||||||
| H00003855-T01 |
KRT7 293T Cell Transient Overexpression Lysate(Denatured) |
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| Abnova Taiwan Corp. | |||||||
| LY401707 |
KRT7 overexpression lysate |
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0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for Cytokeratin 7 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
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|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
![Western Blot: Cytokeratin 7 Lysate [NBL1-12396] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KRT7](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Cytokeratin%207%20Lysate-Western%20Blot-NBL1-12396-img0001.jpg)
