SM3046P Cytokeratin 7 (+ KRT17) antibody

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0.1 mg / €210.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Bovine, Human Cytokeratin 7 C-46

SM3046P

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  • SM3046P
  • SM3046P

Product Description for Cytokeratin 7

Mouse anti Bovine, Human Cytokeratin 7 C-46.
Properties: (+ KRT17)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 7

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
Presentation Purified
Reactivity Bov, Hu
Applications C, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone C-46
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P08729
Immunogen:
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
GeneID:
3855
Property + KRT17
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunoprecipitation.
Immunocytochemistry.
Western Blot: 1 µg/ml
Sample Preparation: Lysis 1% lauryl maltoside fo for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
Positive Control: HeLa Human cell line.
Immunohistochemistry on Paraffin Sections: 20 µg/ml.
Pretreatment: 0.1% pepsin, 30 min, 37°C.
Incubation: 4°C overnight; Chromogen DAB.
Positive tissue: Pancreas.
Background Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Vojtĕsek B, Stasková Z, Nenutil R, Bártková J, Kovarík J, Rejthar A, et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma. 1990;37(3):333-42. PubMed PMID: 1695327.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS, pH~7.4
Preservatives:
15 mM Sodium Azide
State:
Liquid purified Tissue Culture Supernatant (> 95% by SDS-PAGE)
Purified
Species Reactivity
Species reactivity (tested):
Human, Porcine, Bovine.
Specificity
Specificity:
The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). 
Negative Species: Mouse, Rat, Rabbit.
Gene ID 3855

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 7 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 7

Cytokeratin 7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Cytokeratin 7

Cytokeratin 7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 7 (3 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 7 Lysate

Western Blot: Cytokeratin 7 Lysate [NBL1-12396] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT7
  Novus Biologicals Inc.

KRT7 293T Cell Transient Overexpression Lysate(Denatured)

KRT7 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

KRT7 overexpression lysate

KRT7 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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