Product Description for Casein kinase I epsilon
Mouse anti Canine, Human, Mouse, Rat Casein kinase I epsilon 4D7.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for Casein kinase I epsilon
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CKI-epsilon, CKIe, CSNK1E |
| Presentation | Purified |
| Reactivity | Can, Hu, Ms, Rt |
| Applications | E, WB |
| Clonality | Monoclonal |
| Clone | 4D7 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples) |
| Add. information | The lyophilized cell lysates (see below) contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation. |
| Application | ELISA: 0.1 µg/ml. Western blot: Use at 0.5 µg/ml for HRPO/ECL detection. Detects a band at 45kD. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from Untreated A431 cells. |
| Background | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling. |
| Concentration | n/a |
| Protocols | Positive Control: Cell lysate from Untreated A431 cells. Format: Lyophilized cell lysate from Serum starved A431 cells Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 1454 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Casein kinase I epsilon (9 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP302436 |
Casein kinase I epsilon (transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP321884 |
Casein kinase I epsilon (transcript variant 2) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| H00001454-P01 |
Casein kinase I epsilon |
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|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| H00001454-P01-10 |
Casein kinase I epsilon |
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|
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00001454-P01-25 |
Casein kinase I epsilon |
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|
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00001454-Q01-10 |
Casein kinase I epsilon |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001454-Q01-25 |
Casein kinase I epsilon |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P2490 |
Casein kinase I epsilon |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5525 |
Casein kinase I epsilon |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Casein kinase I epsilon (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-09531 |
CK1 epsilon Lysate |
||||||
![Western Blot: CK1 epsilon Lysate [NBL1-09531] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for CSNK1E](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/CK1%20epsilon%20Lysate-Western%20Blot-NBL1-09531-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
| NBL1-09532 |
CK1 epsilon Lysate |
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![Western Blot: CK1 epsilon Lysate [NBL1-09532] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for CSNK1E](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/CK1%20epsilon%20Lysate-Western%20Blot-NBL1-09532-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Casein kinase I epsilon (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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