Product Description for MAPKK 2
Mouse anti Canine, Human, Mouse, Rat MAPKK 2 8E8.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for MAPKK 2
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, MEK2, MKK2 |
| Presentation | Purified |
| Reactivity | Can, Hu, Ms, Rt |
| Applications | E, WB |
| Clonality | Monoclonal |
| Clone | 8E8 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples) |
| Application | Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 µg/ml (protein ELISA). |
| Background | MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase on both threonine and tyrosine residues of the activation motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch. |
| Format |
|
| Specificity |
|
| Gene ID | 5605 |
Accessory Products
Proteins and/or Positive Controls
Proteins for MAPKK 2 (14 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP301827 |
MAPKK 2 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR50344PU-N |
MAPKK 2 (1-400, His-tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
50 µg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR50344PU-S |
MAPKK 2 (1-400, His-tag) |
||||||
|
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
10 µg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| H00005605-P01-10 |
MAPKK 2 |
||||||
|
Human | in vitro transl. | |||||
| Abnova Taiwan Corp. | |||||||
| H00005605-P01-25 |
MAPKK 2 |
||||||
|
|
Human | in vitro transl. | |||||
| Abnova Taiwan Corp. | |||||||
| H00005605-P02-10 |
MAPKK 2 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue | |||||
| Abnova Taiwan Corp. | |||||||
| H00005605-P02-25 |
MAPKK 2 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue | |||||
| Abnova Taiwan Corp. | |||||||
| H00005605-Q01-10 |
MAPKK 2 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005605-Q01-25 |
MAPKK 2 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005605-Q02 |
MAPKK 2 |
||||||
|
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00005605-Q02-25 |
MAPKK 2 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | |||||
| Abnova Taiwan Corp. | |||||||
| P2951 |
MAPKK 2 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2953 |
MAPKK 2 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5583 |
MAPKK 2 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
Positive controls for MAPKK 2 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00005605-T01 |
MAP2K2 293T Cell Transient Overexpression Lysate(Denatured) |
||||||
|
|||||||
| Abnova Taiwan Corp. | |||||||
| LY403069 |
MAP2K2 overexpression lysate |
||||||
|
0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| NBL1-12847 |
MEK2 Lysate |
||||||
![Western Blot: MEK2 Lysate [NBL1-12847] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for MAP2K2](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/MEK2%20Lysate-Western%20Blot-NBL1-12847-img0001.jpg) |
|||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for MAPKK 2 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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