AM00104PU-N PIN1 (incl. pos. control) antibody

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0.1 mg / €450.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Canine, Human, Mouse PIN1 8C10

Product Description for PIN1

Mouse anti Canine, Human, Mouse PIN1 8C10.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for PIN1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1
Presentation Purified
Reactivity Can, Hu, Ms
Applications E, WB
Clonality Monoclonal
Clone 8C10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q13526
Immunogen:
Recombinant Human PIN1
GeneID:
5300
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: 0.1 µg/ml (protein ELISA). 
Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. 
Included Positive Control: Cell lysate from untreated HepG2 cells.
Background PIN1 is a peptidyl-prolyl-cis-trans-isomerase (PPIase) that specifically interacts with serine phosphate-proline or threonine phosphate-proline motifs. Upon binding, PIN1 isomerizes the peptide bond form cis to trans. Known substrates of PIN1 are serveral mitotic phosphoproteins (e.g. cdc25) as well as phosphorylated p53, phosphorylated β-catenin and phosphorylated tau protein. It is assumed that the isomerization of phosphoproteins regulates their biological function.
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Shelf life: one year from despatch.
Format
Purification:
Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Buffer System:
1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with 1 ml H2O (15 min, RT).
Specificity
Specificity:
This antibody specifically recognizes Human PIN1 in cell extracts at 16 kDa.
Species:
Human, Mouse, Dog.
Gene ID 5300

Accessory Products

Proteins and/or Positive Controls

Proteins for PIN1 (9 products)

Catalog No. Species Pres. Purity   Source  

PIN1

PIN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

PIN1

PIN1 Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

PIN1

PIN1 Human Purified > 95 % E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

PIN1

PIN1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

PIN1

PIN1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

PIN1

PIN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PIN1

PIN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PIN1

PIN1 Human
  Abnova Taiwan Corp.

PIN1

SDS-Page: Pin1 Protein [NBC1-18415] - PIN 1, 18.2 kDa (163 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for PIN1 (2 products)

Catalog No. Species Pres. Purity   Source  

Pin1 Lysate

Western Blot: Pin1 Lysate [NBL1-14428] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PIN1
  Novus Biologicals Inc.

PIN1 overexpression lysate

PIN1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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