Product Description for PIN1
Mouse anti Canine, Human, Mouse PIN1 8C10.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for PIN1
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1 |
| Presentation | Purified |
| Reactivity | Can, Hu, Ms |
| Applications | E, WB |
| Clonality | Monoclonal |
| Clone | 8C10 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | ELISA: 0.1 µg/ml (protein ELISA). Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated HepG2 cells. |
| Background | PIN1 is a peptidyl-prolyl-cis-trans-isomerase (PPIase) that specifically interacts with serine phosphate-proline or threonine phosphate-proline motifs. Upon binding, PIN1 isomerizes the peptide bond form cis to trans. Known substrates of PIN1 are serveral mitotic phosphoproteins (e.g. cdc25) as well as phosphorylated p53, phosphorylated β-catenin and phosphorylated tau protein. It is assumed that the isomerization of phosphoproteins regulates their biological function. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 5300 |
Accessory Products
Proteins and/or Positive Controls
Proteins for PIN1 (9 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP302543 |
PIN1 |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| SA6039 |
PIN1 |
||||||
|
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.1 mg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| SA6039X |
PIN1 |
||||||
|
Human | Purified | > 95 % | E. coli |
0.5 mg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| H00005300-P01-10 |
PIN1 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005300-P01-25 |
PIN1 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005300-Q01-10 |
PIN1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005300-Q01-25 |
PIN1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P3447 |
PIN1 |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| NBC1-18415 |
PIN1 |
||||||
|
Protein | ||||||
| Novus Biologicals Inc. | |||||||
Positive controls for PIN1 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-14428 |
Pin1 Lysate |
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| Novus Biologicals Inc. | |||||||
| LY401873 |
PIN1 overexpression lysate |
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![]() |
0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for PIN1 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
![Western Blot: Pin1 Lysate [NBL1-14428] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for PIN1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Pin1%20Lysate-Western%20Blot-NBL1-14428-img0001.jpg)
