Product Description for AKT2 / PKB beta
Mouse anti Human AKT2 / PKB beta 8B7.
Properties: (specific) (incl. pos. control)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (specific) (incl. pos. control)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Properties for AKT2 / PKB beta
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | C, E, WB |
| Clonality | Monoclonal |
| Clone | 8B7 |
| Host | Mouse |
| Isotype | IgG2a |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | specific |
| Isotype control | AM03096PU-N |
| Add. information | Molecular Weight: 60 kDa |
| Application | ELISA: 0.1 µg/ml. Immunohistochemistry. Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated HepG2 cells. |
| Background | Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers. AKT2 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signalling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is activated by a variety of growth factors. AKT2 has been shown to be transcriptionally regulated by MyoD and to activate MyoD-myocyte enhancer binding factor-2 (MEF2) transactivation activity during muscle differentiation. Glycogen synthase kinase 3 (GSK-3) also has been shown to be a downstream target of AKT2. The AKT2 gene is one of the human homologues of v-akt, the transduced oncogene of the AKT8 virus, which induces lymphomas in mice. It has been implicated in breast, ovarian, and pancreatic cancers. |
| Protocols | Positive Control Cell Lysate: HepG2 untreated. Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen, avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are NOT recommended for applications with native proteins such as immunoprecipitation. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch. |
| Format |
|
| Specificity |
|
| Gene ID | 208 |
Accessory Products
Proteins and/or Positive Controls
Proteins for AKT2 / PKB beta (11 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP317733 |
AKT2 / PKB beta |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| H00000208-Q01-10 |
AKT2 / PKB beta |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00000208-Q01-25 |
AKT2 / PKB beta |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P1489 |
AKT2 / PKB beta |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2117 |
AKT2 / PKB beta |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2118 |
AKT2 / PKB beta |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P3836 |
AKT2 / PKB beta |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P3837 |
AKT2 / PKB beta |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4186 |
AKT2 / PKB beta |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4643 |
AKT2 / PKB beta |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| AKT21-C |
AKT2 / PKB beta (WB+ control) |
||||||
|
|
Purified | ||||||
| Alpha Diagnostic Intl. Inc. | |||||||
Positive controls for AKT2 / PKB beta (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07442 |
AKT2 Lysate |
||||||
![Western Blot: AKT2 Lysate [NBL1-07442] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for AKT2](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/AKT2%20Lysate-Western%20Blot-NBL1-07442-img0001.jpg) |
Protein | ||||||
| Novus Biologicals Inc. | |||||||
| LY419836 |
AKT2 overexpression lysate |
||||||
|
0.1 mg /
€495.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for AKT2 / PKB beta (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
Compare Products
You have no items to compare.
My Cart
You have no items in your shopping cart.
