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AM00154PU-N TOP1 / SCL-70 antibody

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0.1 mg / €450.00
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Quick Overview

Mouse anti Human TOP1 / SCL-70 23B11

AM00154PU-N

Product Description for TOP1 / SCL-70

Mouse anti Human TOP1 / SCL-70 23B11.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for TOP1 / SCL-70

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms DNA topoisomerase 1, DNA topoisomerase I, Top-1
Presentation Purified
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone 23B11
Host Mouse
Isotype IgG1
Molecular weight 90 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P11387
Immunogen:
Synthetic peptide conjugated to KLH.
Epitope: amino acids 699-725.
GeneID:
7150
Remarks:
Mol. Weight of Antigen: 90 kDa
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
This product contains a Positive Control for Immunoblot Applications (for details see "Protocols")
Background Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kDa protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then relegated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus, and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.
DNA Topoisomerases catalyze strand breakage of DNA molecules. During DNA cleavage, a tyrosyl residue of the enzyme breaks the DNA strand by forming a covalent phosphotyrosine residue. Rejoining of the DNA strands occurs by a second transesterification reaction.
Protocols Positive Control: HepG2 Untreated Cell Lysate
Formulation:
Lyophilized Cell Lysate from Serum starved HepG2 Cells.
Stability:
Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Application:
The Positive Control cell lysate is recommended for immunoblot applications. 20µl of positive control cell lysate corresponds to ca 80.000 cells.
Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Storage:
Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Shelf life: one year from despatch.
Format
Purification:
Size Exclusion Chromatography
Buffer System:
1ml 2 x PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
PEG and Sucrose
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with 1 ml H2O (15 min, RT).
Specificity
Specificity:
This antibody specifically recognizes Topoisomerase 1 in Western blot applications.
Species:
Human.
Gene ID 7150

Accessory Products

Proteins and/or Positive Controls

Proteins for TOP1 / SCL-70 (7 products)

Catalog No. Species Pres. Purity   Source  

TOP1 / SCL-70
TOP1 / SCL-70 Rabbit Aff - Purified Purified using immobilized mouse monoclonal anti Scl-70. Homogeneity is verified by SDS-PAGE. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with ELISA assays.
  OriGene Technologies GmbH

TOP1 / SCL-70
TOP1 / SCL-70 Human Purified >80.0% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

TOP1 / SCL-70
TOP1 / SCL-70 Human Purified >80.0% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

TOP1 / SCL-70
TOP1 / SCL-70 Human Purified >80.0% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

TOP1 / SCL-70
TOP1 / SCL-70 Human in vitro transl.
  Abnova Taiwan Corp.

TOP1 / SCL-70
TOP1 / SCL-70 Human in vitro transl.
  Abnova Taiwan Corp.

TOP1 / SCL-70
TOP1 / SCL-70 Human Purified > 96 % Insect cells
  BBI Solutions

Positive controls for TOP1 / SCL-70 (1 products)

Catalog No. Species Pres. Purity   Source  

Topoisomerase I Lysate
Western Blot: Topoisomerase I Lysate [NBL1-17194] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TOP1
  Novus Biologicals Inc.

Recommended Secondary Antibodies for TOP1 / SCL-70 (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for TOP1 / SCL-70
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