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SM311F MHC Class I (RT1Aa) antibody

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Mouse anti Rat MHC Class I (RT1Aa) OX-18

SM311F

Product Description for MHC Class I (RT1Aa)

Mouse anti Rat MHC Class I (RT1Aa) OX-18.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for MHC Class I (RT1Aa)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms RT1 class I histocompatibility antigen AA alpha chain
Presentation FITC
Reactivity Rt
Applications F
Clonality Monoclonal
Clone OX-18
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P16391
Immunogen:
Rat spleen glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Isotype control SM20F (for use in rat samples)
Application Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; This product is routinely tested in flow cytometry on rat splenocytes.
Immunohistology: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Background MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Concentration 0.1 mg/ml
General Readings
  1. Fukumoto T, McMaster WR, Williams AF. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol. 1982 Mar;12(3):237-43. PubMed PMID: 6178598.
  2. Bukovský A, Presl J, Zidovský J. Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy. Immunology. 1984 Aug;52(4):631-40. PubMed PMID: 6146566. (Free PMC Article available)
  3. Osawa H, Herrmann T, Diamantstein T. Inhibition of IL 2-dependent proliferation of rat T lymphoblasts by the monoclonal antibody ART62 which reacts with MHC class 1 antigens. J Immunol. 1985 Jun;134(6):3901-6. PubMed PMID: 2580898.
  4. Stet, R.J.M. et al. (1987) U9F4: A monoclonal antibody recognizing a rat polymorphic class I determinant. Transpl. Proc. 19: 3004-3005.
  5. Fujikawa, L. S. et al. (1989) Class Ii antigens on retinal vascular endothelium, pericytes, macrophages and lymphocytes of the rat. Invest. Ophthalmol. Vis. Sci. 30 1): 66-73
  6. Zhai Y, Knechtle S. Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro. Hum Immunol. 1998 Jul;59(7):404-14. PubMed PMID: 9684990.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
State:
Liquid purified IgG
Specificity
Specificity:
This antibody recognises a monomorphic determinant of MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
Species:
Rat.

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