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discontinued

SM2014B FIV p24 gag antibody

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Quick Overview

Mouse anti FIV p24 gag PAK3-2C1

SM2014B

Product Description for FIV p24 gag

Mouse anti FIV p24 gag PAK3-2C1.
Presentation: Biotin
Product is tested for Enzyme Immunoassay.

Properties for FIV p24 gag

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms Feline Immunodeficiency Virus Capsid protein p24
Presentation Biotin
Applications E
Clonality Monoclonal
Clone PAK3-2C1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P16087
Application ELISA.
Background The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2.
Concentration 1.0 mg/ml
General Readings
  1. Lerner DL, Wagaman PC, Phillips TR, Prospero-Garcia O, Henriksen SJ, Fox HS, et al. Increased mutation frequency of feline immunodeficiency virus lacking functional deoxyuridine-triphosphatase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7480-4. PubMed PMID: 7638216. (Free PMC Article available)
  2. Chatterji, U. et al. (2000) Feline Immunodeficiency Virus Vif localises to the nucleus. J. Virol. 74: 2533 - 2540.
  3. Norway RM, Crawford PC, Johnson CM, Mergia A. Thymic lesions in cats infected with a pathogenic molecular clone or an ORF-A/2-deficient molecular clone of feline immunodeficiency virus. J Virol. 2001 Jul;75(13):5833-41. PubMed PMID: 11390584. (Free PMC Article available, 5 images available)
  4. Simon JH, Fouchier RA, Southerling TE, Guerra CB, Grant CK, Malim MH. The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells. J Virol. 1997 Jul;71(7):5259-67. PubMed PMID: 9188594. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Purification:
Affinity chromatography on Protein A
Buffer System:
PBS, pH 7.2 containing 0.05% Sodium Azide
State:
Liquid purified igG
Specificity
Specificity:
This antibody recognises an immunodominant p24 epitope of immunodeficiency virus (FIV) p24 gag. The specific binding epitope recognised by clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding. This antibody can be used as a detection reagent in ELISA assays in combination with AM01001PU-N as a capture reagent. This antibody does not recognise Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus.

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