Product Description for Scramblase 1 / PLSCR1
Mouse anti Human Scramblase 1 / PLSCR1 1E9.
Properties: (N-term) (incl. pos. control)
Presentation: Purified
Product is tested for Western blot / Immunoblot.
Properties: (N-term) (incl. pos. control)
Presentation: Purified
Product is tested for Western blot / Immunoblot.
Properties for Scramblase 1 / PLSCR1
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1, Phospholipid scramblase 1 |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | WB |
| Clonality | Monoclonal |
| Clone | 1E9 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | N-term |
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. |
| Background | Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases. |
| Protocols | Positive Control Cell Lysate HepG2 untreated Size Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 5359 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Scramblase 1 / PLSCR1 (7 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR50617PU-N |
Scramblase 1 / PLSCR1 (1-288, His-tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.25 mg /
€1,070.00
|
||
| OriGene Technologies GmbH | |||||||
| AR50617PU-S |
Scramblase 1 / PLSCR1 (1-288, His-tag) |
||||||
|
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
50 µg /
€400.00
|
||
| OriGene Technologies GmbH | |||||||
| H00005359-P01-10 |
Scramblase 1 / PLSCR1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005359-P01-25 |
Scramblase 1 / PLSCR1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005359-P02 |
Scramblase 1 / PLSCR1 |
||||||
|
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00005359-P02-10 |
Scramblase 1 / PLSCR1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005359-P02-25 |
Scramblase 1 / PLSCR1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Scramblase 1 / PLSCR1 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00005359-T01 |
PLSCR1 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| NBL1-14525 |
Scramblase 1 Lysate |
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![Western Blot: Scramblase 1 Lysate [NBL1-14525] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PLSCR1.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Scramblase%201%20Lysate-Western%20Blot-NBL1-14525-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Scramblase 1 / PLSCR1 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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