discontinued

SP1414P BMF (N-term) antibody

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Rabbit anti Human, Mouse BMF

SP1414P

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  • SP1414P

Product Description for BMF

Rabbit anti Human, Mouse BMF.
Properties: (N-term)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for BMF

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Bcl-2-modifying factor
Presentation Purified
Reactivity Hu, Ms
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q96LC9
Immunogen:
Peptide sequence corresponding to amino acids 2-14 of human Bmf
AA Sequence:
EPSQCVEELEDDV
GeneID:
90427
Property N-term
Application Western blot: 2 µg/ml; this antibody detects a band of approximately 25kDa in HepG2 and 293 cell lysates.
Immunocytochemistry.
Background Bcl-2 modyfying factor is a pro-apoptotic member of the Bcl-2 protein family. Bmf is constitutively expressed in healthy tissues and is sequestered to myosin V motors by association with the dynein light chain 2. Disruption of the actin cytoskeleton is thought to trigger release and activation of Bmf, initiating apoptotic processes downstream.
Concentration 1.0 mg/ml
General Readings
  1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L, Cheney RE, et al. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001 Sep 7;293(5536):1829-32. PubMed PMID: 11546872.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
PBS containing 0.02% Sodium Azide
State:
Liquid purified IgG
Purified
Specificity
Specificity:
This antibody recognises Bcl-2 modifying factor (Bmf).
Species:
Human, Mouse.

Accessory Products

Proteins and/or Positive Controls

Proteins for BMF (7 products)

Catalog No. Species Pres. Purity   Source  

BMF (transcript variant 2)

BMF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

BMF (full length, N-term HIS tag)

BMF Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

BMF (1-129, T7-tag)

BMF Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €1,070.00
  OriGene Technologies GmbH

BMF (1-129, T7-tag)

BMF Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €400.00
  OriGene Technologies GmbH

BMF

BMF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BMF

BMF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BMF

SDS-Page: Bmf Protein [NBC1-28000] - BMF, 15.6 kDa (144aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for BMF (2 products)

Catalog No. Species Pres. Purity   Source  

Bmf Lysate

Western Blot: Bmf Lysate [NBL1-07996] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for BMF Protein
  Novus Biologicals Inc.

BMF overexpression lysate

BMF overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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