Product Description for Interleukin-8 / IL8
Mouse anti Human Interleukin-8 / IL8 BDI670.
Presentation: Azide Free
Product is tested for Enzyme Immunoassay, Functional assay, Western blot / Immunoblot.
Presentation: Azide Free
Product is tested for Enzyme Immunoassay, Functional assay, Western blot / Immunoblot.
Properties for Interleukin-8 / IL8
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor |
| Presentation | Azide Free |
| Reactivity | Hu |
| Applications | E, FN, WB |
| Clonality | Monoclonal |
| Clone | BDI670 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | ELISA. Neutralizing: The antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5µg/ml of this antibody and 10ng/ml of human IL-8. This antibody had no inhibitory effect on MIP 1-beta and RANTES in neutrophil chemotaxis assay. Western blot: Concentration of 0.02-0.1µg/ml will allow visualization of 100ng/lane of human IL-8. |
| Background | Interleukin 8, IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology. |
| Concentration | 1.0 mg/ml (prior to lyophilization) |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for Interleukin-8 / IL8 (43 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP302075 |
Interleukin-8 / IL8 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP720027 |
Interleukin-8 / IL8 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP720029 |
Interleukin-8 / IL8 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP721122 |
Interleukin-8 / IL8 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723246 |
Interleukin-8 / IL8 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
25 µg /
€230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723247 |
Interleukin-8 / IL8 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
25 µg /
€230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723733 |
Interleukin-8 / IL8 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95%, as determined by Coomassie stained SDS-PAGE. |
E. coli |
10 µg /
€195.00
|
|||
| OriGene Technologies, Inc. | |||||||
| PA081 |
Interleukin-8 / IL8 (72 aa) |
||||||
|
Human | > 98 % by SDS-PAGE and HPLC analyses | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| PA081X |
Interleukin-8 / IL8 (72 aa) |
||||||
|
Human | > 98 % by SDS-PAGE and HPLC analyses | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| PA082 |
Interleukin-8 / IL8 (77 aa) (Endothelial) |
||||||
|
Human | > 98 % pure by SDS-PAGE and HPLC analyses | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| PA082X |
Interleukin-8 / IL8 (77 aa) (Endothelial) |
||||||
|
Human | > 98 % pure by SDS-PAGE and HPLC analyses | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| PA1144 |
Interleukin-8 / IL8 (72 aa) |
||||||
|
Human | Purified |
> 98 % Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1144X |
Interleukin-8 / IL8 (72 aa) |
||||||
|
Human | Purified |
> 98 % Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1144XC |
Interleukin-8 / IL8 (72 aa) |
||||||
|
Human | Purified |
> 98 % Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| SA6054 |
Interleukin-8 / IL8 (78 aa) |
||||||
|
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.1 mg /
€250.00
|
||
| OriGene Technologies GmbH | |||||||
| SA6054X |
Interleukin-8 / IL8 (78 aa) |
||||||
|
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.5 mg /
€600.00
|
||
| OriGene Technologies GmbH | |||||||
| H00003576-P01-10 |
Interleukin-8 / IL8 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003576-P01-25 |
Interleukin-8 / IL8 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003576-Q01 |
Interleukin-8 / IL8 |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| H00003576-Q01-10 |
Interleukin-8 / IL8 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003576-Q01-25 |
Interleukin-8 / IL8 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P3641 |
Interleukin-8 / IL8 |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P3642 |
Interleukin-8 / IL8 |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P3993 |
Interleukin-8 / IL8 |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| NBC1-18459 |
Interleukin-8 / IL8 |
||||||
|
Protein | ||||||
| Novus Biologicals Inc. | |||||||
| NBP1-42918-0.1 |
Interleukin-8 / IL8 |
||||||
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| NBP1-42918-0.5 |
Interleukin-8 / IL8 |
||||||
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| NBP1-43217-10 |
Interleukin-8 / IL8 |
||||||
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| NBP1-43217-25 |
Interleukin-8 / IL8 |
||||||
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| NBP1-46089 |
Interleukin-8 / IL8 |
||||||
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| NBP1-74353 |
Interleukin-8 / IL8 |
||||||
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| PRO-50013-0025 |
Interleukin-8 / IL8 (28-99) |
||||||
|
|
Human | Purified | > 95 % 12% SDS-PAGE stained with Coomassie Blue (CB) and peptide fingerprinting by MALDI-TOF mass spectrometry | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50013-0050 |
Interleukin-8 / IL8 (28-99) |
||||||
|
|
Human | Purified | > 95 % 12% SDS-PAGE stained with Coomassie Blue (CB) and peptide fingerprinting by MALDI-TOF mass spectrometry | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50013-0100 |
Interleukin-8 / IL8 (28-99) |
||||||
|
|
Human | Purified | > 95 % 12% SDS-PAGE stained with Coomassie Blue (CB) and peptide fingerprinting by MALDI-TOF mass spectrometry | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50014-0025 |
Interleukin-8 / IL8 (23-99) |
||||||
|
|
Human | Purified | > 95 % 12% SDS-PAGE stained with Coomassie Blue (CB) and peptide fingerprinting by MALDI-TOF mass spectrometry | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50014-0050 |
Interleukin-8 / IL8 (23-99) |
||||||
|
|
Human | Purified | > 95 % 12% SDS-PAGE stained with Coomassie Blue (CB) and peptide fingerprinting by MALDI-TOF mass spectrometry | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50014-0100 |
Interleukin-8 / IL8 (23-99) |
||||||
|
|
Human | Purified | > 95 % 12% SDS-PAGE stained with Coomassie Blue (CB) and peptide fingerprinting by MALDI-TOF mass spectrometry | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50059-0025 |
Interleukin-8 / IL8 (72aa, 28-99) |
||||||
|
Human | Purified | > 95 % 6xHis tag / Immobilized-metal affinity chromatography (IMAC) | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50059-0050 |
Interleukin-8 / IL8 (72aa, 28-99) |
||||||
|
Human | Purified | > 95 % 6xHis tag / Immobilized-metal affinity chromatography (IMAC) | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50059-0100 |
Interleukin-8 / IL8 (72aa, 28-99) |
||||||
|
Human | Purified | > 95 % 6xHis tag / Immobilized-metal affinity chromatography (IMAC) | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50060-0025 |
Interleukin-8 / IL8 (77aa, 23-99) |
||||||
|
Human | Purified | > 95 % 6xHis tag / Immobilized-metal affinity chromatography (IMAC) | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50060-0050 |
Interleukin-8 / IL8 (77aa, 23-99) |
||||||
|
Human | Purified | > 95 % 6xHis tag / Immobilized-metal affinity chromatography (IMAC) | E. coli | |||
| Protein Alternatives | |||||||
| PRO-50060-0100 |
Interleukin-8 / IL8 (77aa, 23-99) |
||||||
|
Human | Purified | > 95 % 6xHis tag / Immobilized-metal affinity chromatography (IMAC) | E. coli | |||
| Protein Alternatives | |||||||
Positive controls for Interleukin-8 / IL8 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00003576-T01 |
IL8 293T Cell Transient Overexpression Lysate(Denatured) |
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| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Interleukin-8 / IL8 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
