AR09511PU-N SORD (1-357, His-tag)

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0.1 mg / €290.00
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Quick Overview

SORD

Product Description for SORD

SORD.
Presentation: Purified

Properties for SORD

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms L-iditol 2-dehydrogenase, Sorbitol dehydrogenase
Presentation Purified
Molecular weight 40.4 kDa
Source E. coli
Specactivity > 0.2 units/mg
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q00796
Purity > 90 % by SDS - PAGE
Background SORD, also known as L-iditol 2-dehydrogenase or SORD1, is a 357 amino acid member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings
  • Baker MA., et al. (2010) Proteomics. 10(3):482-95.
  • Sorger D., et al. (2009) Nucl Med Biol. 36(1):17-27.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human SORD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP
Molecular weight:
(377aa) confirmed by MALDI-TOF
Specific activity:
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37°C.
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 5 mM DTT, 20% glycerol
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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