Sonic hedgehog (SHH) (24-197, His-tag) - AR09512PU-L

Product Description for Sonic hedgehog (SHH)

Sonic hedgehog (SHH).
Presentation: Purified

Immunogen	Swiss Prot Num: Q15465
Purity	> 95 % by SDS - PAGE
Background	SHH, also known as Sonic hedgehog protein, is one of three proteins in the mammalian signaling pathway family called hedgehog. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. It controls cell division of adult stem cells and has been implicated in development of some cancers.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Kolpak A, et al. (2005) J Neurosci. 25(13):3432-41.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human SHH protein, fused to His-tag at C-terminus was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH` Molecular weight: (183aa) confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1 M NaCl Purity: >95% by SDS - PAGE State: Liquid purified protein Purified