D-dopachrome decarboxylase (1-118, His-tag) - AR09530PU-L

Product Description for D-dopachrome decarboxylase

D-dopachrome decarboxylase.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	D-dopachrome tautomerase, DDCT, DDT, Phenylpyruvate tautomerase II
Presentation	Purified
Molecular weight	14.8 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P30046
Purity	> 95 % by SDS - PAGE
Background	DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Arisawa K., et al. (2009) Toxicology. 255(1-2):6-14.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL` Molecular weight: (138aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol Purity: >95% by SDS - PAGE State: Liquid purified protein Purified