AR09537PU-N GOT1 (1-413, His-tag)

Search for all "GOT1"

0.1 mg / €290.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

GOT1

Product Description for GOT1

GOT1.
Presentation: Purified

Properties for GOT1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms Aspartate aminotransferase, Glutamate oxaloacetate transaminase 1, Transaminase A
Presentation Purified
Molecular weight 48.4 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17174
Purity > 95 % by SDS - PAGE
Background Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Totan A., et al. (2006) Clin Chem Lab Med. 44(5):612-5.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
Molecular weight:
(433aa) confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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