discontinued

SM1759PS Follicular Dendritic Cells antibody

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Mouse anti Rat Follicular Dendritic Cells ED5

SM1759PS

Product Description for Follicular Dendritic Cells

Mouse anti Rat Follicular Dendritic Cells ED5.
Presentation: Purified
Product is tested for Frozen Sections.

Properties for Follicular Dendritic Cells

Product Category Primary Antibodies
Quantity 50 µg
Presentation Purified
Reactivity Rt
Applications C
Clonality Monoclonal
Clone ED5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rat spleen cells.
Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0-Ag14 mouse myeloma cell line.
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Application Immunohistochemistry on Frozen Sections: 1/100-1/200.
Background Follicular dendritic cells are cells that facilitate antigen recognition by B cells in follicles. This kind of dendritic cell is not bone marrow derived and is not a kind of blood cell. It is purely a resident of the follicles of secondary lymphoid organs. B cells form germinal centers around follicular dendritic cells in lymphoid organs. Dendritic cells form from monocytes, white blood cells which circulate in the body and, depending on the right signal, can turn into dendritic cells or macrophages. The monocytes in turn are formed from stem cells in the bone marrow.
Concentration 1.0 mg/ml
General Readings
  1. Jeurissen, S.H.M & Dijkstra, C.D. (1986) Characteristics and functional aspects of non lymphoid cells in rat germinal centres, recognised by two monoclonal antibodies ED5 and ED6. Eur. J. Immunol. 16: 562-568.
  2. Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, et al. Follicular dendritic cell (FDC) precursors in primary lymphoid tissues. J Immunol. 1998 Feb 1;160(3):1078-84. PubMed PMID: 9570519.
  3. Yamate J, Kuriyama T, Kawashima M, Nakanishi M, Ide M, Kuwamura M, et al. Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma. Virchows Arch. 2002 Jun;440(6):616-26. Epub 2002 Feb 13. PubMed PMID: 12070602.
  4. Köhler, C.N. (2009) The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth Muscle Cells, Reticular Cells, and Follicular Dendritic Cells. J Histochem Cytochem. Nov 3. [Epub ahead of print]
  5. Ohmori, Y. et al. (2001) Repeated Antigen Challenge Modulates Expression of Follicular Dendritic Cell (FDC) Related Molecule in Draining Lymph Nodes Acta Histochemica et Cytochemica 34: 265-73.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS containing 0.09% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Species Reactivity
Species reactivity (tested):
Rat. Does not react with Mouse.
Specificity
Specificity:
ED5 staining reveals a web-like pattern in B cell follicles, representing the follicular dendritic cells. Mesangial cells in the kidney are also ED5-positive.

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