AR09180PU-N GFP (1-238)

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0.1 mg / €300.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

GFP

Product Description for GFP

GFP.
Presentation: Purified

Properties for GFP

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms GFP-Tag, Green fluorescent protein
Presentation Purified
Molecular weight 26.8 kDa
Source E. coli
Species (Protein) A. victoria
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P42212
Purity > 95 % by SDS - PAGE
Background GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Concentration 1 mg/ml (determined by Bradford assay)
General Readings
  • Millan MI, et al. (2009). Medicina, 69(3):370-4.
  • Marshall J, et al. (1995). Neuron, 14(2): 211-5.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
Molecular weight:
Confirmed by MALDI-TOF (238 aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Endotoxin level:
< 1.0 EU per 1 µg of protein (determined by LAL method )
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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