discontinued

AR09181PU-N SLC4A4 / NBCE1 (Isoform 2) (1-424, His-tag)

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SLC4A4 / NBCE1

Product Description for SLC4A4 / NBCE1

SLC4A4 / NBCE1.
Presentation: Purified

Properties for SLC4A4 / NBCE1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms Electrogenic sodium bicarbonate cotransporter 1, NBC, NBC1, Na(+)/HCO3(-) cotransporter, Sodium bicarbonate cotransporter 1, Solute carrier family 4 member 4
Presentation Purified
Molecular weight 51.5 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y6R1
Purity > 90 % by SDS - PAGE
Background Sodium bicarbonate cotransporters (NBCs) mediate the coupled movement of sodium and bicarbonate ions across the plasma membrane of many cells. This is an electrogenic process with an apparent stoichiometry of 3 bicarbonate ions per sodium ion. NBC1 has been identified as a key player for regulation of intracellular pH in several cell types. NBC1 Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings
  1. Rickmann M, Orlowski B, Heupel K, Roussa E. Distinct expression and subcellular localization patterns of Na+/HCO3- cotransporter (SLC 4A4) variants NBCe1-A and NBCe1-B in mouse brain. Neuroscience. 2007 May 25;146(3):1220-31. Epub 2007 Apr 12. PubMed PMID: 17433553.
  2. Zhu Q, Azimov R, Kao L, Newman D, Liu W, Abuladze N, et al. NBCe1-A Transmembrane Segment 1 Lines the Ion Translocation Pathway. J Biol Chem. 2009 Mar 27;284(13):8918-29. doi: 10.1074/jbc.M806674200. Epub 2009 Jan 21. PubMed PMID: 19158093. (Free PMC Article available, 11 images available)
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human NBC1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTE NVEGKPSNLG ERGRARSSTF LRVVQPMFNH SIFTSAVSPA AERIRFILGE EDDSPAPPQL FTELDELLAV DGQEMEWKET ARWIKFEEKV EQGGERWSKP HVATLSLHSL FELRTCMEKG SIMLDREASS LPQLVEMIVD HQIETGLLKP ELKDKVTYTL LRKHRHQTKK SNLRSLADIG KTVSSASRMF TNPDNGSPAM THRNLTSSSL NDISDKPEKD QLKNKFMKKL PRDAEASNVL VGEVDFLDTP FIAFVRLQQA VMLGALTEVP VPTRFLFILL GPKGKAKSYH EIGRAIATLM SDEVFHDIAY KAKDRHDLIA GIDEFLDEVI VLPPGEWDPA IRIEPPKSLP SSDKRKNMYS GGENVQMNGD TPHDGGHGGG GHGDCEELQR TGRFCGGLIK DIKRKAPFFA SDFYDALNIQ
Molecular weight:
(460 aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 7.5) containing 0.5 mM DTT, 0.1 mM PMSF, 10% glycerol
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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