AR09272PU-L VEGF-A (His-tag)

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0.25 mg / €1,070.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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VEGF-A

AR09272PU-L

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  • AR09272PU-L

Product Description for VEGF-A

VEGF-A.
Presentation: Purified

Properties for VEGF-A

Product Category Proteins & Growth Factors
Quantity 0.25 mg
Synonyms VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Presentation Purified
Molecular weight 16.3 kDa
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q00731
Purity > 90 % by SDS - PAGE
Background VEGF-A, also known as vascular endothelial growth factor, belong to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Gerber H., et al. (2002) Nature. 417: 954-958.
Shibuya M., et al. (2006) J Biochem Mol Biol. 39:469-478.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant mouse VEGF-A protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
Biological activity:
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml.
Activity Assay
1.Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast )
2.Maintenance Condition: RPMI 1640 containing 10% FBS
3.Assay medium: serum free RPMI 1640
4.Cell density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5.Incubation time: 24 hr (after sample treatment)
6.Concentration range: 1 pg/ml – 1 ug/ml
7.Detection method : BrdU assay
Molecular weight:
(141 aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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