Product Description for Vimentin
Vimentin.
Presentation: Purified
Presentation: Purified
Properties for Vimentin
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | VIM |
| Presentation | Purified |
| Molecular weight | 57,000 |
| Species (Protein) | Bovine |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Purity | > 98 % determined by SDS gelelectrophoresis |
| Add. information | Isoelectric Point: pI 5.3 |
| Application | Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. |
| Background | Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. |
| Concentration | 1,0 mg/ml |
| Protocols | Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM sodium phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). |
| General Readings |
|
| Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Source | Bovine lens |
| Description |
|
| Format |
|
Compare Products
You have no items to compare.
My Cart
You have no items in your shopping cart.
