Product Description for Cytokeratin 18
Cytokeratin 18.
Presentation: Purified
Presentation: Purified
Properties for Cytokeratin 18
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18 |
| Presentation | Purified |
| Molecular weight | 45 kDa |
| Source | Liver |
| Species (Protein) | Bovine |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 95 % (determined by SDS gelelectrophoresis) |
| Add. information | Isoelectric Point: pI 5.4 |
| Application | Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. |
| Background | Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Concentration | 1.0 mg/ml |
| Protocols | Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For Immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). See References 2 and 3 for more details. |
| General Readings |
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| Storage | Store at 2-8°C (lyophilized) and at -20°C (reconstituted). Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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