AM09123SU-N Plakophilin 3 / PKP3 antibody

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5 ml / €300.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Bovine, Human, Mouse, Xenopus Plakophilin 3 / PKP3 PKP3-270.6.2

AM09123SU-N

Product Description for Plakophilin 3 / PKP3

Mouse anti Bovine, Human, Mouse, Xenopus Plakophilin 3 / PKP3 PKP3-270.6.2.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Plakophilin 3 / PKP3

Product Category Primary Antibodies
Quantity 5 ml
Synonyms Plakophilin-3
Presentation Supernatant
Reactivity Bov, Hu, Ms, Xen
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone PKP3-270.6.2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y446
Immunogen:
Recombinant protein (E. coli) of human plakophilin 3
GeneID:
11187
Application Immunoblotting: 1/500 ECL method.
Immunofluorescence.
Immunohistochemistry on frozen sections: Ready-to-use.
Background Plakophilin 3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
General Readings
  1. Schmidt A, Langbein L, Prätzel S, Rackwitz HR, Franke WW: Plakophilin 3 – A novel cell-type specific plaque protein. Differentiaton 64(5):291-306 (1999)
  2. Vasioukin V, Bowers E, Bauer C, Degenstein L, Fuchs E: Desmoplakin is essential in epidermal sheet formation. Nature Cell Biology 3, 1076-1085 (2001)
  3. Akat K, Mennel HD, Kremer P, Gassler N, Bleck CK, Kartenbeck J. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 2003 Oct;106(4):337-47. Epub 2003 Jul 5. PubMed PMID: 12845453.
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
State:
Liquid culture supernatant
Supernatant
Specificity
Specificity:
This antibody reacts to Plakophilin 3.
Positive tissues and culture cells:
- stratified squamous epithelia: palmar, epidermis, scalp skin
- stratified epithelia: tongue, vagina, esophagus
- transitional epithelia: bladder
- non-stratified epithelia: colon, duodenum, pancreas,
several glands
- non-epithelial: reticulum cells of lymph node follicle
- HaCaT, MCF7, HeLa, HT-29, CaCo 2, Caski, BMGE, MDCK and other
Negative tissues and cell lines:
- liver, heart, skeletal muscle, endothelia (vessels)
- PLC, RD, SV80, HL60, Raji, Jok1, Glioma, K562, 3T3L1, A6, PtK2
Species:
Human, Bovine, Mouse, Xenopus laevis.
Gene ID 11187

Accessory Products

Proteins and/or Positive Controls

Proteins for Plakophilin 3 / PKP3 (3 products)

Catalog No. Species Pres. Purity   Source  

Plakophilin 3 / PKP3

Plakophilin 3 / PKP3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Plakophilin 3 / PKP3

Plakophilin 3 / PKP3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Plakophilin 3 / PKP3

Plakophilin 3 / PKP3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Plakophilin 3 / PKP3 (2 products)

Catalog No. Species Pres. Purity   Source  

PKP3 overexpression lysate

PKP3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

Plakophilin 2 Lysate

Western Blot: Plakophilin 2 Lysate [NBL1-14467] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PKP2
  Novus Biologicals Inc.
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