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AM09135SU-N Desmoglein-1 antibody

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0.5 ml / €480.00
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Quick Overview

Mouse anti Human Desmoglein-1 Dsg1-P23

AM09135SU-N

Product Description for Desmoglein-1

Mouse anti Human Desmoglein-1 Dsg1-P23.
Presentation: Supernatant
Product is tested for Frozen Sections, Paraffin Sections, Western blot / Immunoblot.

Properties for Desmoglein-1

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms CDHF4, Cadherin family member 4, DG1, DGI, DSG1, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen
Presentation Supernatant
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone Dsg1-P23
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q02413
Immunogen:
Recombinant Human polypeptide Desmoglein 1 (extracellular part EII, EIII and EIV)
GeneID:
1828
Application Immunoblotting (Western). 
Immunohistochemistrry on Frozen Sections: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended (See Ref.1)
Immunohistochemistry on Paraffin Embedded Sections (After Microwave treatment): For better staining on paraffin sections preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media is recommended.
Recommneded Dilutions: 1/1000 for 1h at RT for Immunohistochemistry (Dilute immediately before use in PBS).
Background Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.
Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus.
Desmoglein 1 is a 165kD glycoprotein that is a core component of the desmosome cell-cell junction between epithelial cells. Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.
General Readings
  1. Schäfer S, Stumpp S, Franke WW. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 1996 May;60(2):99-108. PubMed PMID: 8641550.
  2.  Akat K, Mennel H-D, Kremer P, Gassler N, Bleck CKE, Kartenbeck J: Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol 106, 337-347 (2003). 
  3.  Kurzen H, Moll I, Moll R, Schäfer S, Simics E, Masayuki A, Wheelock MJ, Franke WW: Compositionally different desmosomes in the various compartments of the human hair follicle. Differentiation 63, 295-304 (1998)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Hybridoma Culture Supernatant (Concentrate)
Supernatant
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
In Immunoblots of cytoskeletal preparations from Human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1.
In immunolocalisation studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. 
Cross reaction with Desmoglein 2 and 3 was not detected.
Gene ID 1828

Accessory Products

Proteins and/or Positive Controls

Proteins for Desmoglein-1 (2 products)

Catalog No. Species Pres. Purity   Source  

Desmoglein-1
Desmoglein-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Desmoglein-1
Desmoglein-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for Desmoglein-1 (1 products)

Catalog No. Species Pres. Purity   Source  

DSG1 293T Cell Transient Overexpression Lysate(Denatured)
DSG1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

Recommended Secondary Antibodies for Desmoglein-1 (11 products)

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Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
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Mouse IgG (H+L chain) antibody
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
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